Melanocyte growth supplement

Manufactured by ScienCell

Sourced in United States

Melanocyte growth supplement is a cell culture media supplement designed to support the growth and proliferation of melanocytes in vitro. The product contains a proprietary formulation of growth factors, hormones, and other essential components required for the optimal growth of melanocyte cells.

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Lab products found in correlation

Penicillin streptomycin, by ScienCell (2 mentions) Fetal bovine serum (fbs), by ScienCell (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution, by ScienCell (1 mentions) Sk mel 28, by American Type Culture Collection (1 mentions) A2058, by American Type Culture Collection (1 mentions) Sk mel 2, by American Type Culture Collection (1 mentions) N acetylcysteine nac, by Merck Group (1 mentions) Raw 264.7 murine macrophage, by American Type Culture Collection (1 mentions) B16f10 murine melanoma cells, by American Type Culture Collection (1 mentions) A375 human melanoma cells, by American Type Culture Collection (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions)

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Growth supplement (13 citations)

5 protocols using melanocyte growth supplement

Establishing Melanoma and Melanocyte Cell Lines

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CMM cell lines SK-MEL-28, M14, A375, and A875 were provided by the central laboratory of Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College and cultured in Dulbecco’s Modified Eagle’s medium (Gibco, USA) with 10% FBS (Gibco; USA) under 37°C and 5% CO₂. Human epidermal melanocytes (MC) were separated from healthy donors’ prepuce. Prepuce was cut into strips and digested with 0.25% trypsin at 4°C overnight. The next day, the epidermis was separated from the dermis with fine forceps, incubated with 0.25% trypsin for 10 min, and neutralized with 10% FBS in DMEM. The collection was changed to suspension by repeated pipetting, filtered (100 μm filter), centrifuged at 1200 rpm for 4 min. The isolated melanocytes were resuspended in MelM media (ScienCell, USA) containing melanocyte growth supplement (ScienCell, USA) and penicillin/streptomycin solution (ScienCell, USA) under 37°C and 5% CO2.

Wang Y., Song H., Miao Q., Wang Y., Qi J., Xu X, & Sun J. (2022). PLA2G6 Silencing Suppresses Melanoma Progression and Affects Ferroptosis Revealed by Quantitative Proteomics. Frontiers in Oncology, 12, 819235.

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Melanoma Cell Line Cultivation Protocol

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A2058, 1205Lu, HT114, SK-Mel2, and SK-Mel28 were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). SK-Mel119, SK-Mel187, C8161, and UACC903 were kindly provided by Dr. Suzie Chen. All the cells were cultured in 1x RPMI1640 containing 10% fetal bovine serum (FBS) except for HT144 cell line grown in McCoy’s 5 A containing 10% FBS. Human primary melanocytes were purchased from Coriell Institute for Medical Research (Camden, NJ, USA) and cultured in MelM medium containing melanocyte growth supplement (ScienCell Research Laboratories, Carlsbad, CA, USA) with 5% FBS. 1% Penicillin/Streptomycin was added to the media used. All cell lines were maintained at 37 °C in a humidified 5% CO₂ incubator. Mycoplasma negativity and cell authentication were tested on a regular basis at the Core Facility (The Biospecimen Repository and Histopathology Service) of Rutgers University.

Shin S.S., Jeong B.S., Wall B.A., Li J., Shan N.L., Wen Y., Goydos J.S, & Chen S. (2018). Participation of xCT in melanoma cell proliferation in vitro and tumorigenesis in vivo. Oncogenesis, 7(11), 86.

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Evaluating ZnO Nanoparticle Cytotoxicity in Melanocytes

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Human epidermal melanocytes (HEMs) were purchased from Sciencecell (Carlsbad, CA, USA) and maintained in melanocyte medium containing 1% melanocyte growth supplement (Carlsbad, CA, USA), 0.5% fetal bovine serum (Carlsbad, CA, USA), and 1% penicillin/streptomycin (Carlsbad, CA, USA) at 37 °C in a humidified atmosphere of 5% CO₂. The culture medium was replaced every other day, and cells were passaged upon reaching approximately 90% confluence. HEMs were cultured in diluted ZnO NP working solution containing 2.5, 5.0, 7.5, or 10 µg/ml ZnO NPs for 24, 48 and 72 h.
N-acetylcysteine (NAC) (Sigma-Aldrich; 20 mM), the N-acetyl derivative of cysteine, inhibits ROS in HEMs were treated with 5.0 µg/ml ZnO NPs for 24, 48 and 72 h.

Wang P., Hu G., Zhao W., Du J., You M., Xv M., Yang H., Zhang M., Yan F., Huang M., Wang X., Zhang L, & Chen Y. (2022). Continuous ZnO nanoparticle exposure induces melanoma-like skin lesions in epidermal barrier dysfunction model mice through anti-apoptotic effects mediated by the oxidative stress–activated NF-κB pathway. Journal of Nanobiotechnology, 20, 111.

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Culturing Diverse Cell Lines for Research

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B16F10 murine melanoma cells, 4T1 murine mammary carcinoma cells, HUVEC human endothelial cells, RAW264.7 murine macrophages, YUMM1.7 murine melanoma cells, A375 human melanoma cells, C32 human melanoma cells and murine Lewis Lung Carcinoma (LLC) cells designated LL/2 (LLC1) were obtained from the American Type Culture Collection (ATCC; cat. no. CRL-1642). Human Dermal Fibroblast (HDF) were obtained from ScienCell Research Laboratories, Inc. and cultured in 2% FBS in growth medium supplemented with Fibroblast Growth Supplements from ScienCell Research Laboratories, Inc. YUMM1.7 cells were cultured in DMEM F-12 medium in presence of 10% FBS, 1% NEAA and 1% Pen-Strep. 4T1 cells were cultured in RPMI-1640 medium in the presence of 5% FBS, 1% Pen-Strep and 1% sodium pyruvate. HUVECs were cultured in VCBM media plus Endothelial Cell Growth Kit. Β16F10, RAW264.7, C32, A375 and LLC cells were cultured in DMEM plus 10% FBS 1.0% Pen-Strep and 1.0% sodium pyruvate. Human primary melanocytes were obtained from ScienCell Research Laboratories, Inc. and cultured in Melanocyte Media containing 0.5% FBS and Melanocyte Growth Supplement.

Caron J.M., Han X., Lary C.W., Sathyanarayana P., Remick S.C., Ernstoff M.S., Herlyn M, & Brooks P.C. (2023). Targeting the secreted RGDKGE collagen fragment reduces PD‑L1 by a proteasome‑dependent mechanism and inhibits tumor growth. Oncology Reports, 49(2), 44.

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Inhibition of ZnO Nanoparticle Cytotoxicity

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HEMs were purchased from Sciencecell (Carlsbad, CA, USA) and maintained in melanocyte medium containing 1% melanocyte growth supplement (Carlsbad, CA, USA), 0.5% fetal bovine serum (Carlsbad, CA, USA), and 1% penicillin/streptomycin (Carlsbad, CA, USA) at 37°C in a humidified atmosphere of 5% CO2. The culture medium was replaced every other day, and cells were passaged upon reaching approximately 90% confluence. NAC (Sigma-Aldrich; 20 mM), the N-acetyl derivative of cysteine, inhibits ROS. NAC-treated HEMs were cultured in diluted ZnO NP working solution containing 2.5, 5, 7.5, or 10 µg•mL -1 ZnO NPs for 24, 48, or 72 h.

Wang P., Hu G., Zhao W., Du J., You M., Xv M., Yang H., Zhang M., Yan F., Huang M., Wang X., Zhang L., & Chen Y. (2021). Continuous ZnO nanoparticle exposure induces melanoma-like skin lesions in epidermal barrier dysfunction model mice through anti-apoptotic effects mediated by the oxidative stress–activated NF-κB pathway.

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