Genomic DNA extraction from dry seeds was performed following the method of Dang and Chen (2013 (link)). A Nano-Drop 2000c spectrophotometer (Nano Drop Technologies, USA) was used to evaluate the quality and concentration of all DNA. DNA samples were diluted to 20 ng/μL and PCR conditions were applied: 94°C for 1 min, 30 cycles of 30 s at 94°C, 50°C for 1.0 min, 72°C for 1.5 min, and 1 cycle at 72°C for 10 min. PCR products and DNA molecular weight marker (Promega, Madison, WI) were separated on a 1.2% TAE-agarose gel and image was captured on a Gel Logic 200 Imaging System (Kodak, Rochester, NY).
Dna molecular weight marker
Manufactured by Promega
Sourced in United States
A DNA molecular weight marker is a standardized mixture of DNA fragments of known sizes used to determine the approximate size of DNA samples in gel electrophoresis. It serves as a reference to compare and estimate the size of unknown DNA samples.
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Lab products found in correlation
2 protocols using dna molecular weight marker
1
Genomic DNA Extraction from Dry Seeds
2
Gel Electrophoresis of PCR and RT-PCR Products
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The PCR and RT-PCR products were analyzed on a 1% agarose gel containing ethidium bromide (0.5μg/ml). Primarily, a 1% agarose gel was prepared by weighing 0.5gram agarose in a 250ml flask and loaded 50ml of 0.5X TAE to it. Then 1µl red gels added and pour in to tank and then place comb. In addition, 1µl loading dye (buffer) was mixed with 5µl PCR product and load these samples in to the prepared well in the gel and run at 120 volt for about 25 minutes in parallel with DNA molecular weight marker (Promega Biosciences Inc. in San Luis Obispo, CA, USA) in electrophoresis apparatus. Finally, The DNA bands were visualized by UV transilluminator and the expected sizes were identified against DNA molecular weight markers (Promega Biosciences Inc. in San Luis Obispo, CA, USA).
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