Human recombinant EGF and human recombinant TNF were purchased from Peprotech (Rocky Hill, NJ, USA). Gefitinib was kindly provided by AstraZeneca (Tokyo, Japan). TAPI-1 was from Calbiochem (San Diego, CA, USA). The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): Total EGFR antibody (#4267), phospho-EGFR (Y1068) antibody (#3777), total AKT antibody (#9272), phospho-AKT (S473) antibody (#9271), total ERK1/2 antibody (#9102), phospho-ERK1/2 antibody (#4370), phospho-MKK3/6 antibody (#9236), total p38 MAPK antibody (#9212), phospho-p38 MAPK antibody (#9211), TACE antibody (#3976), and β-actin antibody (#4970). Meanwhile, antibodies specific to total MKK 3/6 (sc-13069) was purchased from Santa Cruz Biotechnology, Dallas, TX, USA. Cetuximab was purchased from Showa University Hospital (Shinagawa, Tokyo, Japan). Materials used for western blot analysis were from Bio-Rad Laboratories (Richmond, CA, USA). Dulbecco’s modified Eagle medium (DMEM) and all other materials were purchased from Sigma Chemical (St. Louis, MO, USA) unless otherwise stated. All pharmacological inhibitors and agonists were dissolved in DMSO and added to the medium with a final DMSO concentration of <0.1%.
Total p38 mapk antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Total p38 MAPK antibody is a research-use only product that detects the total levels of the p38 mitogen-activated protein kinase (MAPK) protein. The p38 MAPK is a key signaling molecule involved in various cellular processes. This antibody can be used in techniques such as Western blotting to measure the total expression of the p38 MAPK protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Total akt antibody, by Cell Signaling Technology (2 mentions)
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
β actin antibody, by Cell Signaling Technology (1 mentions)
Phospho erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Recombinant human egf, by Thermo Fisher Scientific (1 mentions)
Recombinant human tnf, by Thermo Fisher Scientific (1 mentions)
Total egfr antibody, by Cell Signaling Technology (1 mentions)
Phospho akt s473 antibody, by Cell Signaling Technology (1 mentions)
Total erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Phospho p38 mapk antibody, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle medium dmem, by Merck Group (1 mentions)
Gefitinib, by AstraZeneca (1 mentions)
Aβ1 42 peptide, by Merck Group (1 mentions)
Phospho gsk 3β ser9 antibody, by Cell Signaling Technology (1 mentions)
Ginsenoside rh1, by Merck Group (1 mentions)
Carboxy h2dcfda, by Merck Group (1 mentions)
1 1 1 3 3 3 hexafluoro 2 propanol, by Merck Group (1 mentions)
Total gsk3 β antibody, by Cell Signaling Technology (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
2 protocols using total p38 mapk antibody
1
Investigating EGFR and Cell Signaling
2
Preparation and Characterization of Aβ Oligomers
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Ginsenoside Rh1 (C 36 H 62 O 9 ), Aβ 1-42 peptide, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), Fluorescein diacetate (FDA), propidium iodide (PI), and carboxy-H 2 DCF-DA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hoechst 33,342 was obtained from Thermo Fisher Scientific (Manassas, VA, USA). Phospho-protein kinase B (Akt; Ser473) antibody, total Akt antibody, phospho-GSK-3β (Ser9) antibody, total GSK-3β antibody, phospho-p38 mitogen-activated protein kinase (MAPK; Thr180/Tyr182) antibody, and total p38 MAPK antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). LY294002 was obtained from Bioscience (Franklin Lakes, NJ, USA), and PD98059 was obtained from Calbiochem (San Diego, CA, USA).
Aβ oligomers were prepared as previously described [37] (link). The Aβ 1-42 peptide was dissolved in HFIP to make 1 mM of Aβ monomer. Aliquots (100 µL) of Aβ monomer solution were evaporated completely in a hood for 12 h and stored at -80 • C. They were dissolved in 11 µL of dimethyl sulfoxide (≥99.9 %) and 540 µL of phosphate-buffered saline (PBS) and then stored for 24 h (at 4 • C) to make oligomers. We used 1~4 µM of Aβ oligomers.
Aβ oligomers were prepared as previously described [37] (link). The Aβ 1-42 peptide was dissolved in HFIP to make 1 mM of Aβ monomer. Aliquots (100 µL) of Aβ monomer solution were evaporated completely in a hood for 12 h and stored at -80 • C. They were dissolved in 11 µL of dimethyl sulfoxide (≥99.9 %) and 540 µL of phosphate-buffered saline (PBS) and then stored for 24 h (at 4 • C) to make oligomers. We used 1~4 µM of Aβ oligomers.
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