Immulite2000 total testosterone kit

FGF16 was purchased from PeproTech (Rocky Hill, NJ). Immulite2000 Total Testosterone kit was purchased from Sinopharm (Hangzhou, Zhejiang, China). Culture medium (M199, DMEM and F12) and Click‐iT EdU (EdU) imaging kit were purchased from Invitrogen (Carlsbad, CA). EDS was purchased from Pterosaur Biotech (Hangzhou, Zhejiang, China). Antibody information was listed in Table S1. Animals were purchased from Shanghai Laboratory Animal Center (Shanghai, China). The use of animals was approved by the Animal Care and Use Committee of Wenzhou Medical University.

Atrazine was purchased from Sigma (St. Louis, MO, United States). SYBR Green qPCR Kit and Protein Assay Kit were purchased from Takara (Otsu, Japan). Trizol Kit was purchased from Invitrogen (Carlsbad, CA, United States). Immulite2000 Total Testosterone Kit was purchased from Sinopharm (Hangzhou, China). Antibody information was presented in Supplementary Table S1. Adult male and female Sprague-Dawley rats were purchased from Shanghai Laboratory Animal Center (Shanghai, China). The experimental protocol for animal toxicity study was approved by Wenzhou Medical University Laboratory Animal Ethics Committee and in conformity with procedures described in the Guide for the Care and Use of Laboratory Animals published by NIH (United States). The detailed methods are listed in Supplementary Data Sheet S1.