The following antibodies were used in this study: mAb Flag [F3165, (RRID:AB_259529 ); Sigma, St Louis, MO, USA], mAb Flag‐HRP [A8592 (RRID:AB_439702); Sigma], mAb GFP [A11120 (RRID:AB_221568); Thermo Fisher Scientific Inc., Waltham, MA, USA], mAb PSD95 [MA1‐045 (RRID:AB_325399); Thermo], mAb PSD93 [Neuromab, 75‐057 (RRID:AB_2277296)], mAb GluN1 [32‐0500 (RRID:AB_2533060); Thermo], pAb IQsec2 (gift from Professor James Casanova, University of Virginia, RRID:AB_2636960)], mAb Adam22 [Neuromab, 75‐083 (RRID:AB_10675128)], pAb Arc [Synaptic systems, 156003 (RRID:AB_887694)], mAb Kir2.3 [Neuromab, 75‐069 (RRID:AB_2130742)].
Mab flag
Manufactured by Merck Group
Sourced in United States
The MAb-Flag is a laboratory instrument used for the detection and purification of proteins. It utilizes monoclonal antibodies specific to the FLAG tag, a widely used protein tag, to capture and isolate proteins of interest from complex mixtures. The core function of the MAb-Flag is to facilitate the efficient isolation and analysis of FLAG-tagged proteins in a research or analytical setting.
Automatically generated - may contain errors
Lab products found in correlation
Mab ha, by Merck Group (4 mentions)
Mab flag hrp, by Merck Group (2 mentions)
Pab flag, by Merck Group (2 mentions)
Mab β actin, by Merck Group (2 mentions)
Lsm 780, by Zeiss (2 mentions)
Alexa 555, by Thermo Fisher Scientific (1 mentions)
Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions)
Mab cyclin b1, by Santa Cruz Biotechnology (1 mentions)
Mab β actin ac 15, by Merck Group (1 mentions)
Mab ul44, by Merck Group (1 mentions)
Streptomyces griseus, by Merck Group (1 mentions)
Mab lamin a c, by Abcam (1 mentions)
Pab lamin a c pser22, by Antibodies-online (1 mentions)
Gapdh mab, by Abcam (1 mentions)
Actin hrp, by Merck Group (1 mentions)
Srebp1 mab, by Santa Cruz Biotechnology (1 mentions)
α tubulin mab, by Merck Group (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
11 protocols using mab flag
1
Antibodies for Neuroscience Research
2
Antibody Characterization in Cell Biology
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Antibodies used in this study: mAb-HA (Clone 7, H9658, Sigma-Aldrich); pAb-HA (Signalway Eurogentec, College Park, MD, USA); mAb-HA-HRP (12013819001, Roche, Basel, Switzerland); mAb-Flag (F1804, Sigma-Aldrich); mAb-Flag-HRP (A8592, Sigma-Aldrich); pAb-Histone H3 (PA5-17869, Invitrogen); mAb-Lamin A/C (sc-7292, Santa Cruz, Dallas, TX, USA); pAb-Aldolase (sc-12065, Santa Cruz); chicken Fc (chicken Fc fragment, 003-000-008, Jackson ImmunoResearch, Ely, UK); mAb-UL53.01 (kindly provided by Stipan Jonjic and Tihana Lenac Rovis, University of Rijeka, Rijeka, Croatia); anti-mouse Alexa 488 (A-11001, Thermo Fisher Scientific), anti-rabbit Alexa 555 (A-21428, Thermo Fisher Scientific).
3
Antibodies in Cell Biology Research
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Antibodies used in this study: mAb-HA (Clone 7, H9658, Sigma Aldrich); pAb-HA (Signalway Eurogentec, College Park, MD, USA); mAb-Flag (F1804, Sigma Aldrich); pAb-Flag (F7425, Sigma Aldrich); mAb-Myc (ab9106, Abcam, Cambridge, UK); pAb-Strep (2-1507-001, IBA Lifesciences, Göttingen, Germany); mouse Fc (mouse Fc fragment, 015-000-008, Dianova, Hamburg, Germany); rabbit Fc (rabbit Fc fragment, 011-000-008, Dianova); mAb-Orf27, mAb-Orf24, mAb-UL50.01, mAb-UL97.01 (all kindly provided by Stipan Jonjic and Tihana Lenac Rovis, University of Rijeka, Rijeka, Croatia); mAb-BFRF1 (kindly provided by Alberto Faggioni, Sapienza University of Rome, Rome, Italy); PepAS-M53 (kindly provided by Walter Muranyi, Universitätsklinikum Heidelberg, Heidelberg, Germany); PepAS-M50 (kindly provided by Zsolt Ruzsics, Virology, University of Freiburg, Freiburg, Germany); mAb-β-Actin (A5441, Sigma Aldrich); mAb-mIE1 (Anti-m123/IE1, MCMV, CROMA101, Center for Proteomics, Rijeka, Croatia); pAb-p32 (sc-48795, Santa Cruz Biotechnology, Dallas, TX, USA); anti-mouse Alexa 488 (A-11001, Thermo Fisher Scientific), anti-rabbit Alexa 555 (A-21428, Thermo Fisher Scientific).
4
Antibody Panel for Protein Analysis
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The following polyclonal (pAb) and monoclonal (mAb) antibodies were used: mAb-UL97 (clone 1C4/0.2, produced and kindly provided by Dr. T. Lenac/Prof. S. Jonjic, Dept. Histology and Embryology, Univ. Rijeka, Croatia), pAb-UL97 (06-09, kindly provided by Prof. D. M. Coen, Harvard Medical School, Boston, MA, USA), mAb-UL44 (BS 510, kindly provided by Prof. B. Plachter, Univ. Mainz, Mainz, Germany), mAb-pp65 (65-33, kindly provided by Prof. W. J. Britt, UAB, Birmingham, AL, USA), mAb-β-actin (AC-15, Sigma-Aldrich), mAb-cyclin B1 (sc-7393, Santa Cruz; GNS11, Thermo Fisher Scientific, Waltham, MA, USA), pAb-cyclin B1 (sc-752, Santa Cruz), mAb-cyclin T1 (sc-271348, Santa Cruz), pAb-cyclin T1 (sc-10750, Santa Cruz), pAb-Fc (rabbit Fc fragment, Jackson ImmunoResearch Laboratories, Bar Harbor, ME, USA), mAb-Flag (M2, Sigma-Aldrich), pAb-Flag (F7425, Sigma Aldrich), and mAb-HA (Clone 7, Sigma-Aldrich). The following fluorescent dye-conjugated secondary antibodies were applied in immunofluorescence analyses: Alexa 488-conjugated goat anti-rabbit IgG (H+L) and Alexa 555-conjugated goat anti-mouse IgG (H+L) (Life Technologies, Carlsbad, CA, USA).
5
Zebrafish Embryo Protein Analysis
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Zebrafish embryos were collected 24 and 48 hours post-microinjection and dechorionated with 1% pronase (protease from Streptomyces griseus; Sigma-Aldrich, USA) in E3 medium at 37 °C in agarose-coated Petri dishes. Embryos were deyolked (55 mM NaCl, 1.8 mM KCl, 1.25 mM NaHCO3 containing protease inhibitors (cOmplete, Roche)), lysed in 2x SDS-sample buffer (2 µl per embryo) for 5 min at 95 °C and subjected to standard western blot analysis (12 µl per lane equals ~6 embryos) using the following antibodies: pAb-UL97 (kindly provided by D.M. Coen, Harvard Medical School, Boston, MA, USA), mAb-GFP (Roche), mAb-FLAG (Sigma-Aldrich), and mAb-α-tubulin (Abcam).
6
Comprehensive Antibody Panel for Cytomegalovirus
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Antibodies used in this study are mAb-IE1p72, mAb-MCP, mAb-pp28 (all kindly provided by William Britt, University of Alabama, Birmingham, AL, USA), mAb-UL44 (kindly provided by Bodo Plachter, University of Mainz, Mainz, Germany), mAb-UL50.01, mAb-UL53.01, mAb-UL97.01 (all kindly provided by Stipan Jonjic and Tihana Lenac Rovis, University of Rijeka, Rijeka, Croatia), mAb-β-Actin (A5441, Sigma Aldrich, St. Louis, MO, USA), mab-lamin A/C (ab108595, Abcam, Cambridge, UK), pAb-lamin A/C pSer22 (ABIN1532183, Antibodies online, Aachen, Germany), pAb-Pin1 (10495-1-AP, Proteintech, Rosemont, IL, USA), anti-Cytomegalovirus-Alexa Fluor 488 (IE1/MAB810X, Merck, Darmstadt, Germany), mAb-HA (Clone 7, H9658, Sigma Aldrich), mAb-Flag (F1804, Sigma Aldrich), mAb-emerin (Sc-25284, Santa Cruz, Dallas, TX, USA), anti-mouse Alexa 555 (A-21422, Thermo Fisher Scientific, Waltham, MA, USA) and anti-rabbit Alexa 488 (A-11008, Thermo Fisher Scientific).
7
Immunofluorescent Staining of Nuclear Proteins
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Cells were seeded into 24-well plates containing glass circle cover slips (Thermo Fisher) prior to experimentation. Cells were transfected when ~50% confluent as indicated. PBS-MC buffer was used for all washes and as a diluent for all solutions. Cells were fixed with 4% paraformaldehyde in PBS-MC for 21-min. Cells were permeabilized for 5-min in 0.2% Triton-X in PBS, then placed in blocking solution (5% Normal Goat Serum and 5% BSA in PBS) for 1-hr. Primary and secondary antibodies were diluted in 5% normal goat serum in PBS-MC. Cells were incubated with primary Ab's O/N at 4 °C. Cells were washed with PBS-MC and then incubated in secondary Ab's for 1-hr. Next, cells were counter stained with a 1:20,000 solution of DAPI in PBS for 10-min. Finally, cover slips were washed once with PBS-MC and mounted on slides. Images were captured with a Zeiss LSM 780 confocal microscopy and analyzed with Zen 2011 LSM 780 software. Ab's used include: (i) fibrillarin mAb (Gene Tex: GTX24566); B23 mAb (ProteinTech: 60096-1); Flag mAb (Sigma: F3165); fibrillarin rabbit-Alexa 488 conjugate (Cell Signaling: C13C3).
8
Protein Immunoprecipitation and Western Blot
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Whole-cell extracts were collected via the lysis of 1 × 107 cells in 500 μL 1× IP Lysis Buffer for 30 min on ice and clarified by centrifugation. Protein extracts were incubated at 4 °C overnight with FLAG mAb (Sigma Aldrich, #F1804) or control immunoglobulin G, and precipitated proteins were captured with Pierce Protein A/G Agarose for 6 h at 4 °C. After twice washing in IP Lysis Buffer with a complete protease inhibitor mixture, bound proteins were eluted in 5 × SDS loading buffer and analyzed by Western blot.
9
Antibody Panel for Cell Analysis
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Several antibodies were used throughout this study: pan 14-3-3 pAb (Santa Cruz, K19 sc-629), K8 mAb (DSHB, 1h5), C-Cadherin mAb (DSHB, 6B6), pan Cadherin pAb (Santa Cruz, H-300 sc-10733), Vinculin mAb (Millipore, MAB3574), K19 mAb (Progen, 61010), pan keratin mAb (Sigma Aldrich, C2562), Actin-HRP (Sigma-Aldrich,A3854), GAPDH mAb (Abcam, mAbcam 9484), GFP mAb (Invitrogen, A-11120), GFP mAb (Santa Cruz, B-2 sc-9996), mCherry pAb (BioVision, 5993-100), and FLAG mAb (Sigma-Aldrich, F1804). The 1h5 anti-XCK1(8) monoclonal antibody developed by Michael Klymkowsky and 6B6 anti-C-cadherin monoclonal antibody developed by Barry Gumbiner were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the Department of Biology, University of Iowa (Iowa City, IA 52242).
10
Antibody Panel for EMT Analysis
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The following monoclonal (mAb) and polyclonal (pAb) primary antibodies were used for western blot and IHC: NFYA pAb (1:500; Santa Cruz, no. sc-10779), NFYA mAb (1:500; Santa Cruz, no. sc-17753), E-Cadherin mAb (1:1000; BD, no. 610181), Vimentin mAb (1:1000; BD, no. 550513), SNAI 1 mAb (1:500; Santa Cruz, no. sc-28199), Flag mAb (1:1000; Sigma, no. F3165), FASN Rabbit mAb (1:1000 for western blot, 1:100 for IHC; CST, no. 3180), ACACA Rabbit mAb (1:1000 for western blot, 1:100 for IHC; CST, no. 3676), CPT1A Rabbit mAb (1:1000; Abcam, no. Ab234111), ACADVL mAb (1:1000; Santa Cruz, no. sc-376239), SREBP1 mAb (1:500; Santa Cruz, no. sc-13551), CD36 pAb (1:500; Santa Cruz, no. sc-9154), Keratin 14 pAb (1:500; BioLegend, no. 905304), Keratin 8 mAb (1:500; BioLegend, no. 904804), and α-Tubulin mAb (1:2000; Sigma, no. T5168).
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