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Mak077

Manufactured by Merck Group
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Sourced in United States

The MAK077 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use, but its core function is not available in this factual and unbiased description.

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Lab products found in correlation

Mak078, by Merck Group (2 mentions) Mak066, by Merck Group (1 mentions) A113 2, by Nanjing Jiancheng (1 mentions) Mak052, by Merck Group (1 mentions) Mak055, by Merck Group (1 mentions) A111 2, by Nanjing Jiancheng (1 mentions) Mak080, by Merck Group (1 mentions) Mak006, by Merck Group (1 mentions) Mak116, by Merck Group (1 mentions)

5 protocols using mak077

1

Serum UA and XO Determination

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The serum UA concentrations and XO levels in the serum and liver were determined with standard diagnostic kits (MAK077, MAK078, Sigma, USA) according to the manufacturer’s instructions. Tissue homogenate samples were prepared with a 0.9% sterile saline solution.
Li L., Wang D., Wang X., Bai R., Wang C., Gao Y, & Anastassiades T. (2019). N-Butyrylated hyaluronic acid ameliorates gout and hyperuricemia in animal models. Pharmaceutical Biology, 57(1), 717-728.
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2

Colorimetric Analysis of SUA and Creatinine

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SUA concentrations and creatinine levels were separately determined using colorimetric method (#MAK077, #MAK080, Sigma, USA).
Cao L., Zhao T., Xue Y., Xue L., Chen Y., Quan F., Xiao Y., Wan W., Han M., Jiang Q., Lu L., Zou H, & Zhu X. (2022). The Anti-Inflammatory and Uric Acid Lowering Effects of Si-Miao-San on Gout. Frontiers in Immunology, 12, 777522.
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3

Serum Uric Acid and Xanthine Oxidase Assay

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Serum UA concentration was determined by enzymatic-colorimetric method, using a standard diagnostic kit (MAK077, Sigma-Aldrich, USA) according to manufacturer's instructions.
XO levels in serum and liver were determined using a standard diagnostic kit (MAK078, Sigma-Aldrich, USA) according to manufacturer's instructions.
Li L., Teng M., Liu Y., Qu Y., Zhang Y., Lin F, & Wang D. (2017). Anti-Gouty Arthritis and Antihyperuricemia Effects of Sunflower (Helianthus annuus) Head Extract in Gouty and Hyperuricemia Animal Models. BioMed Research International, 2017, 5852076.
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4

Serum Uric Acid Quantification

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The serum uric acid concentration was determined by an enzymatic colorimetric method, using a standard diagnostic kit (MAK077) purchased from Sigma-Aldrich (St. Louis, MO, USA) according to manufacturer’s instructions.
Mohamed Isa S.S., Ablat A, & Mohamad J. (2018). The Antioxidant and Xanthine Oxidase Inhibitory Activity of Plumeria rubra Flowers. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(2), 400.
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5

Serum and Hepatic Lipid Profiling

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Serum parameters including total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol levels were measured with the commercial kits (A111-2, A112-2, and A113-2; Jiancheng). Serum chemistry tests including alanine aminotransferase, aspartate aminotransferase, creatine kinase, lactate dehydrogenase, urea nitrogen, creatinine, uric acid, albumin, and total protein were performed with the commercial kits (MAK052, MAK055, MAK116, MAK066, MAK006, MAK080, MAK077; 09753 and 71285-M; Sigma–Aldrich). Triglyceride was extracted using 5% NP-40 solution in liver and heated at 90 °C for twice. Then it was cooled down to room temperature and centrifuged at 12,000 rpm for 2 min to collect transparent supernatant. The serum and hepatic levels of triglyceride were measured using TG kit (K952; BioVision).
Yu J., Hu Y., Sheng M., Gao M., Guo W., Zhang Z., Wang D., Wu X., Li J., Chen Y., Zhao W., Liu C., Cui X., Chen X., Zhao C., Chen H., Xiao J., Chen S., Luo C., Xu L., Gu X, & Ma X. (2023). Selective PPARγ modulator diosmin improves insulin sensitivity and promotes browning of white fat. The Journal of Biological Chemistry, 299(4), 103059.
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