Size measurements were made at 25°C with a Zetasizer Nano S DLS apparatus (Malvern Instruments, Worcestershire, UK). After the ultracentrifugation procedure abovementioned, each sample was diluted in PBS and was read three times in the Zeta Sizer Nano S (Zen 1600) to evaluate the diameter of the collected particles. A histogram of the percentage of particles with specific diameters was calculated using DTS (nano) 7.03 software (Malvern Instruments). Data represent an average of 3 measurements for each sample.
Zetasizer nano s dls apparatus
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer Nano S is a dynamic light scattering (DLS) apparatus manufactured by Malvern Panalytical. The core function of the Zetasizer Nano S is to measure the size of particles and molecules in a liquid sample by analyzing the Brownian motion of the particles.
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Lab products found in correlation
2 protocols using zetasizer nano s dls apparatus
1
Particle Size Analysis by DLS
2
Protein Size Distribution and Hydrodynamic Diameter Measurements
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The size distribution and hydrodynamic diameter measurements were performed at 25°C with a Zetasizer Nano-S DLS apparatus (Malvern Instruments Ltd., UK) using a low-volume quartz cuvette. Protein samples were diluted to ~2 mg/mL in 25 mM Tris-HCl and 100 mM sodium chloride (pH 7.5). For each sample, 7 to 10 runs 10 s in length were performed. Size distributions and hydrodynamic diameters were calculated using the multiple narrow distribution analysis models of the DTS v.7.11 software (Malvern Instruments Ltd., UK).
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