Comet assays were performed using the OxiSelect Comet Assay Kit (Cell Biolabs) following the manufacturer’s recommendations. Briefly, 750 cells were mixed with 10 volumes of melted Oxiselect Comet Agarose at 37°C and pipetted onto the Oxiselect Comet Slide. Cells were lysed with pre-chilled lysis buffer at 4°C for 1 h, incubated with pre-chilled alkaline buffer at 4°C for 30 min, and then analyzed by electrophoresis under alkaline condition. Cells were washed with pre-chilled water, fixed with 70% ethanol for 5 min, stained with Vista Green DNA dye, and visualized by Nikon ECLIPSE TE2000-U microscope with X-Cite 120 Fluorescence Illumination System. Images were captured using MetaVue Imaging (Molecular Devices). The comet tail was measured by Comet Assay IV software (Perceptive Instruments).
Metavue imaging
Manufactured by Molecular Devices
The MetaVue Imaging is a laboratory equipment designed for high-performance imaging applications. It provides advanced imaging capabilities for various research and analytical tasks.
Automatically generated - may contain errors
2 protocols using metavue imaging
1
Comet Assay for DNA Damage Analysis
2
Comet Assay for DNA Damage Analysis
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!