To assess InsP binding to Arr1, an intrinsic tyrosine fluorescence quenching assay was performed (Arr1 has 14 Tyr residues/monomer), as done previously (Wilson and Copeland, 1997 (
link)). 300 nM Arr1 in 500 µL of Heparin Equilibration Buffer was mixed in a
quartz cuvette (PerkinElmer, Waltham, MA) with mini stir bar mixing on low and temperature maintained at 20 °C. A PerkinElmer LS55, coupled to an
Isotemp 3016S (Fisher Scientific, Pittsburgh, PA) to regulate temperature, was used to measure fluorescence of samples after serial addition of InsP stocks diluted in the same buffer as the Arr1. Device settings for the scans were Ex/Em range of wavelengths of 275/290–350 nm. A slit width of 7 nm was used with a scan rate of 100 nm per min. Every scan was repeated 3 times, and the emission peak at 305 nm was used for the tyrosine emission wavelength. Percent quench values were adjusted for increasing dilution with additional ligand added 5 µL at a time. Curves were fit to the means of the replicates with one site binding accounting for ligand depletion.
Sander C.L., Luu J., Kim K., Furkert D., Jang K., Reichenwallner J., Kang M., Lee H.J., Eger B.T., Choe H.W., Fiedler D., Ernst O.P., Kim Y.J., Palczewski K, & Kiser P.D. (2021). Structural evidence for visual arrestin priming via complexation of phosphoinositols. Structure (London, England : 1993), 30(2), 263-277.e5.