Light microscope digital camera

Samples of kidneys, pancreas, eye cornea, and retina were taken from the experimental rats, fixed in neutral buffered formalin for 48 h, and processed for hematoxylin-eosin histopathological examination using routine methods as described previously [27 (link)]. Briefly, the collected tissues of kidneys, pancreas, eye cornea, and eye retina from rats were immersed in 4% paraformaldehyde for 48 h and then dehydrated step by step using a gradient of ethanol (50, 70, 80, 90, 95, and 100%). Tissue samples were immersed in xylene for 30 min and incubated in paraffin at 65°C overnight. Once embedded in wax, the samples were cut serially into 5 μm thick sections using a microtome (Leica Biosystem, Wetzlar, Germany) and spread over microscopy slides. The sections were deparaffinized with fresh xylene for 10 min, rehydrated with a gradient of ethanol (100, 90, 80, and 70%), and then washed three times with deionized double-filtered distilled water. The sections were analyzed using hematoxylin-eosin staining and examined with a light microscope digital camera (Nikon Instruments, Tokyo, Japan).

Haematoxylin and eosin (H&E) staining of femur and organ (liver, spleen and kidney) tissue, and Giemsa staining of femur tissue, were performed as described in our previous research (Li et al. 2020 (link)). A light microscope digital camera (Nikon Instruments, Tokyo, Japan) was used for histological examinations.

Collected tissues were immerged in 4% paraformaldehyde for 48 h and then dehydrated in gradient ethanol (50%, 70%, 80%, 90%, 95%, and 100%) step by step. Samples were immerged in xylene for 30 min and incubated with first paraffin at 65°C overnight. After embedding in wax, tissues were cut into serial sections at 5 μm thickness using microtome (Leica, Germany) and spread over microscopy slides. Sections were deparaffinized with fresh xylene for 10 min, hydrated with gradient ethanol (100%, 90%, 80%, and 70%), and then washed with double distilled water for three times. The sections were analyzed via haematoxylin and eosin staining (H&E staining) [26 (link)] and examined by a light microscope digital camera (Nikon Instruments, Tokyo, Japan).

The collected kidney tissue was immersed in 4% paraformaldehyde for 48 h and then dehydrated step by step using a gradient of ethanol (50%, 70%, 80%, 90%, 95%, and 100%). Samples were immersed in xylene for 30 min and incubated in paraffin at 65°C overnight. Once embedded in wax, the samples were cut serially into 5 μm thick sections using a microtome (Leica, Germany) and spread over microscopy slides. The sections were deparaffinized with fresh xylene for 10 min, rehydrated with a gradient of ethanol (100%, 90%, 80%, and 70%), and then washed three times with DD water. The sections were analyzed via hematoxylin and eosin (H&E) staining and examined with a light microscope digital camera (Nikon Instruments, Tokyo, Japan).