Fei tecnai g2 f20 s twin transmission electron microscope

In this study, the experimental instruments included: (1) RmTracer-200-HS portable Raman spectrometer combined with a 785 nm excitation wavelength diode-stabilized stimulator (Opto Trace Technologies, Inc., Mountain View, CA, USA); (2) The FEI Tecnai G2 F20 S-TWIN transmission electron microscope (FEI Company, Hillsboro, OR, USA); (3) Vortex-Genie 2/2T vortex mixer (Shanghai Ling early Environmental Protection Instrument Co., Ltd., Shanghai, China). Moreover, the experimental reagents included: (1) Sildenafil (99.8% purity, Sigma-Aldrich, Beijing, China); (2) methanol (chromatographically pure, Amethyst Chemicals, Beijing, China); (3) cocktail (Shanghai Baxter Liquor Co., Ltd., Shanghai, China). In addition, the OTR 202 nanomaterials and OTR 103 produced by Opto Trace Technologies, Inc. (SuZhou, China) were also used in this study.

Transmission electron microscopy (TEM) was used to examine the mechanism of TP4 action on H. pylori, overall cell morphology, TP4 translocation into H. pylori, and binding of TP4 to internal constituents. H. pylori overnight cultures from BHI-blood agar plates were collected, and 0.1 OD inocula were incubated with TP4, Amoxicillin, and/or PBS (PBS only: negative control). Following drug exposure, H. pylori cells were collected and successively washed twice in PBS by centrifugation and resuspension. The cells were then fixed and processed for TEM using previously described methods [67 (link), 68 (link)]. The morphology of the cells was observed using an FEI Tecnai G2 F20 S-TWIN transmission electron microscope (FEI Company, Hillsboro, OR) operating at 80 keV [69 (link)] at low-power magnification (X1100), and the cell wall ultrastructure was observed at high-power magnification (X2, 500/5,000/7,000, or X15, 000). Each grid was examined under the same settings. Images were recorded with a 4MP SPOT Insight charge-coupled-device (CCD) camera.