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Anti tgfbr1

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Anti-TGFBR1 is a lab equipment product that targets the transforming growth factor beta receptor type-1 (TGFBR1) protein. It can be used in various research applications to study the TGFBR1 protein and its role in cellular processes.

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Lab products found in correlation

Anti actin, by Beyotime (1 mentions) Rpmi 1640 medium, by GenePharma (1 mentions) Anti junb, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Cell lysis buffer, by Beyotime (1 mentions) Anti tfpi2, by Santa Cruz Biotechnology (1 mentions) Anti tgfbr2, by Santa Cruz Biotechnology (1 mentions) Anti smad7, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

TGFBR1 (10 citations)

2 protocols using anti tgfbr1

1

Culturing Kidney Cell Lines for Experiments

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The human kidney carcinoma cell line, 786-O, and the human embryonic kidney cell line, 293T, were purchased from American Type Culture Collection (ATCC). Cells were cultured in RPMI-1640 medium (Genepharma, Shanghai, China) supplemented with 10% fetal bovine serum (Invitrogen) in 5% CO2 at 37°C. The primary antibodies anti-TGFBR1 and anti-JunB were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and primary antibody anti-Actin was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Secondary antibodies anti-rabbit IgG and anti-mouse IgG were purchased from Beyotime and Santa Cruz, respectively.
He H., Wang L., Zhou W., Zhang Z., Wang L., Xu S., Wang D., Dong J., Tang C., Tang H., Yi X, & Ge J. (2015). MicroRNA Expression Profiling in Clear Cell Renal Cell Carcinoma: Identification and Functional Validation of Key miRNAs. PLoS ONE, 10(5), e0125672.
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2

Immunoprecipitation of TFPI2, SMAD7, TGFBR1, TGFBR2

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Cells were lysed with cell lysis buffer (#P0013, Beyotime). Whole cell lysates were obtained after centrifugation, and protein concentration was determined. The protein extracts were diluted to 1 μg/μl in phosphate buffer saline (PBS) and then incubated with 1 μg of the indicated antibody and 60 μl protein A agarose beads for 2 h at 4 °C. The antibodies for IP include anti-TFPI2 (#sc-48380; Santa Cruz), anti-SMAD7 (#sc-101152, Santa Cruz), anti-TGFBR1 (#sc-518018, Santa Cruz), and anti-TGFBR2 (#sc-17792, Santa Cruz). IgG was used as a negative control. Immunocomplexes obtained by centrifugation were separated by SDS-PAGE, and immunoblotting was performed as previously described.
Guan G., Xie J., Dai Y, & Han H. (2022). TFPI2 suppresses the interaction of TGF-β2 pathway regulators to promote endothelial–mesenchymal transition in diabetic nephropathy. The Journal of Biological Chemistry, 298(3), 101725.
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