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Specific sandwich elisa

Manufactured by Thermo Fisher Scientific
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The Specific Sandwich ELISA is a laboratory equipment product designed for quantitative detection and measurement of a specific analyte in a sample. It utilizes the principles of enzyme-linked immunosorbent assay (ELISA) to capture the target analyte between two antibodies, one immobilized on a solid surface and the other conjugated with an enzyme. The enzymatic reaction generates a measurable signal proportional to the concentration of the analyte in the sample.

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Specific quantitative sandwich ELISA kits Species-specific sandwich ELISA Sandwich ELISA Specific ELISA ELISAs

2 protocols using specific sandwich elisa

1

Cytokine Quantification by ELISA

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Cytokine levels in the culture medium were determined by a specific sandwich ELISA (Biosource, Camarillo, CA, USA) in 96-well microtiter plates coated with specific cytokine antibodies as previously described [20 (link),41 (link)].
Lee G.L., Wu J.Y., Tsai C.S., Lin C.Y., Tsai Y.T., Lin C.S., Wang Y.F., Yet S.F., Hsu Y.J, & Kuo C.C. (2016). TLR4-Activated MAPK-IL-6 Axis Regulates Vascular Smooth Muscle Cell Function. International Journal of Molecular Sciences, 17(9), 1394.
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2

Cytokine and Protein Analysis by ELISA and Western Blot

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Cytokine levels in the culture supernatants and serum were determined in microtiter plates (96 wells) by a specific sandwich ELISA (Biosource or eBioscience) as previously described. 17, (link)18 (link) Western Blot Analysis
Cellular proteins were resolved with 5% to 20% SDS-PAGE and transferred to nitrocellulose membranes. They were blotted with specific antibodies as previously described. 19 (link)
Wang Y.F., Hsu Y.J., Wu H.F., Lee G.L., Yang Y.S., Wu J.Y., Yet S.F., Wu K.K, & Kuo C.C. (2016). Endothelium-Derived 5-Methoxytryptophan Is a Circulating Anti-Inflammatory Molecule That Blocks Systemic Inflammation. Circulation research, 119(2).
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