Column chromatography was performed using silica gel 60, 230–400 mesh ASTM (Merck, 0.040–0.063mm). Aluminium supported silica gel 60 F254 plates 20 x 20cm (absorbent thickness: 0.25 mm) were used for thin layer chromatography (TLC) (Merck, Germany). Preparative thin layer chromatography (PTLC) (Merck, Germany) silica gel 60 F254 glass plates 20 x 20cm (absorbent thickness: 0.50 mm) were used for separation of compounds. IR spectrum was recorded using a Perkin-Elmer Spectrum 400 FT-IR Spectrometer with spectroscopic grade chloroform as the solvent. 1D- and 2D-NMR spectra were recorded in chloroform CDCl3 (Merck, Germany) using JEOL ECA 400 MHz NMR spectrometer. The LCMS-IT-TOF spectra were recorded on a UFLC Shimadzu Liquid Chromatography with a SPD-M20A diode array detector coupled to a IT-TOF mass spectrometer. UV spectra were recorded using a Shimadzu 1650 PC UV-Vis Spectrophotometer with spectroscopic grade methanol (CH3OH) as solvent. All solvents were of analytical grade and were distilled prior to use.
1650 pc uv vis spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The 1650 PC UV-Vis Spectrophotometer is a laboratory instrument used to measure the absorption or transmittance of light in a sample across a range of ultraviolet and visible wavelengths. It is designed to quantify the concentration of chemical species in a solution by measuring the amount of light absorbed or transmitted.
Automatically generated - may contain errors
Lab products found in correlation
Spectrum 400 ft ir spectrometer, by PerkinElmer (2 mentions)
230 400 mesh astm, by Merck Group (1 mentions)
Eca 400 mhz nmr spectrometer, by JEOL (1 mentions)
Evans blue dye, by Merck Group (1 mentions)
8400 s ftir, by Shimadzu (1 mentions)
Drx 400 spectrometer, by Bruker (1 mentions)
8101m ft ir, by Shimadzu (1 mentions)
D5000 diffractometer, by Siemens (1 mentions)
Leo q06, by Zeiss (1 mentions)
Mira3 microscope, by TESCAN (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Spectrometer, by Bruker (1 mentions)
Cd3od, by Merck Group (1 mentions)
Agilent 6530 accurate mass q tof lc ms system, by Agilent Technologies (1 mentions)
Merck silica gel 60 f254 plates, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
Sephadex lh 20, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
6 protocols using 1650 pc uv vis spectrophotometer
1
Chromatographic Purification and Analysis
2
Pulmonary Transvascular Permeability Measurement
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Evans blue-albumin tracing for pulmonary transvascular permeability was conducted as previously described [3 (link)]. The right jugular vein of ketamine-xylazine anesthetized mice was injected with 8 µl/g body weight Evans Blue–albumin (40 mg/ml BSA containing 1% Evans blue dye, Sigma) and left to circulate for 45 min. A 2-min PBS perfusion into the right ventricle was used to wash out the intravascular Evans Blue. Lungs were excised, homogenized in PBS (1 ml), and extracted using formamide (2 ml) at 60°C overnight. A Shimadzu 1650 PC UV-vis spectrophotometer (Kyoto, Japan) was used to measure Evans Blue content at OD620 and normalized against body weight.
3
Spectroscopic Characterization of 2Z-HYCA Nanocomposite
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Fourier transform infrared spectra (FT-IR) were obtained from transmission measurements (Shimadzu 8400S FTIR, 4000–400 cm−1, 40 Scans) using KBr pellets prepared with a Shimadzu MHP-1 mini hand press. The background correction was performed with a pure KBr pellet, and the samples were measured at 10% in 90% KBr. UV–vis spectroscopy was performed on a Shimadzu 1650pc UV–vis spectrophotometer for analysis of 4-nitrophenol. Cross-polarized magic angle spinning nuclear magnetic resonance 27Al (104.1 MHz), 13C (100.5 MHz), and 29Si (79.4 MHz) spectra of the 2Z-HYCA@650 °C nanocomposite were recorded on a Bruker DRX-400 spectrometer with a magic angle spin probe and 4 mm ZrO2 rotor 27Al signals were referenced to a 0.5 M aqueous solution of aluminum nitrate. 13C and 29Si signals were referenced to tetramethylsilane (TMS).
4
Multitechnique Characterization of Nanoparticles
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The FTIR spectrum of samples was obtained using Shimadzu 8101M FTIR (Kyoto, Japan) at ambient conditions. The samples were mixed with potassium bromide (KBr) and pressed to form a pellet. SEM (Jeol JSM-6390 SEM) and EDX detector (Oxford Link SATW ultrathin window) equipped SEM were applied to obtain SEM micrographs and semi-quantitative elemental analysis. The samples were coated with conductive elements to avoid any interferences with the imaging and analyzing process. A TEM microscopy (Zeiss Leo q06) was used to provide proper TEM images from the NPs at 200 kV accelerating voltage. The NPs were dispersed in solution with low concertation and a small amount of the dispersed NPs (10 µL) was poured on the TEM grid and allowed to dry. A Zetasizer Nano ZS90 was applied to measure the hydrodynamic size and zeta potential of NPs at RT. The UV-vis spectrum of the synthesized NPs was recorded using a spectrophotometer (Shimadzu 1650 PC UV-vis spectrophotometer. Kyoto, Japan). Siemens D5000 diffractometer (Aubrey, Texas, United States) was applied to record the X-ray diffraction (XRD) pattern of the synthesized NPs.
5
Characterization of Microbubble Formulations
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Fourier transform
infrared (FT-IR) spectra of the samples were obtained using a Shimadzu
8101 M FT-IR (Shimadzu, Kyoto, Japan) at a wavenumber range of 4000–400
cm–1. Proton nuclear magnetic resonance (1HNMR) spectra were obtained at 25 °C using an NMR (400 mHz)
Bruker spectrometer (Bruker, Ettlingen, Germany). The samples were
prepared in deuterated chloroform and dimethyl sulfoxide solvents
(DMSO-d6). Ultraviolet–visible
(UV–vis) spectra were taken on a Shimadzu 1650 PC UV–vis
spectrophotometer (Shimadzu, Kyoto, Japan). The morphology of the
MBs was studied by a Zeiss-EM10C-100 kV transmission electron microscope
(TEM, Germany) and optical microscopy. The zeta potential, size distribution,
average diameter, and polydispersity index (PDI) of the MSs were measured
with a Malvern Nano ZS90 Zetasizer (Malvern Instruments, UK) at 25
°C. The employed ultrasound devices (UTS) were a device working
at an acoustic frequency of 1.7 MHz and an acoustic intensity of 1–600
mW cm–2 (Sahand University Technology, Tabriz, Iran)
and another device working at an acoustic frequency of 1.5 MHz and
an acoustic intensity of 35 mW cm–2 (Exogen Ultrasound).
The field emission scanning electron microscopy (FE-SEM) was carried
out using a MIRA3 microscope (TESCAN, Czech) to determine the morphology
of the cells and MSs.
infrared (FT-IR) spectra of the samples were obtained using a Shimadzu
8101 M FT-IR (Shimadzu, Kyoto, Japan) at a wavenumber range of 4000–400
cm–1. Proton nuclear magnetic resonance (1HNMR) spectra were obtained at 25 °C using an NMR (400 mHz)
Bruker spectrometer (Bruker, Ettlingen, Germany). The samples were
prepared in deuterated chloroform and dimethyl sulfoxide solvents
(DMSO-d6). Ultraviolet–visible
(UV–vis) spectra were taken on a Shimadzu 1650 PC UV–vis
spectrophotometer (Shimadzu, Kyoto, Japan). The morphology of the
MBs was studied by a Zeiss-EM10C-100 kV transmission electron microscope
(TEM, Germany) and optical microscopy. The zeta potential, size distribution,
average diameter, and polydispersity index (PDI) of the MSs were measured
with a Malvern Nano ZS90 Zetasizer (Malvern Instruments, UK) at 25
°C. The employed ultrasound devices (UTS) were a device working
at an acoustic frequency of 1.7 MHz and an acoustic intensity of 1–600
mW cm–2 (Sahand University Technology, Tabriz, Iran)
and another device working at an acoustic frequency of 1.5 MHz and
an acoustic intensity of 35 mW cm–2 (Exogen Ultrasound).
The field emission scanning electron microscopy (FE-SEM) was carried
out using a MIRA3 microscope (TESCAN, Czech) to determine the morphology
of the cells and MSs.
6
Isolation and Characterization of Bioactive Compounds from M. cinnamomea
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Analytical and preparative TLC was carried out on Merck 60 F 254 silica gel plates (absorbent thickness: 0.25 and 0.50 mm, respectively) (Merck, Germany). Column chromatography (CC) was performed using silica gel (230-400 mesh, ASTM) (Merck, Germany) and Sephadex LH-20 (Sigma-Aldrich, USA). IR spectra were recorded using a Perkin-Elmer Spectrum 400 FT-IR Spectrometer. NMR spectra were acquired in CD 3 OD (Merck, Germany) using a JOEL ECA 400 MHz NMR Spectrometer. LCMS-IT-TOF spectra were obtained using an Agilent 6530 Accurate-Mass Q-TOF LC/MS system. UV spectra were recorded using a Shimadzu 1650 PC UV-V is Spectrophotometer. All solvents were of analytical grade and were distilled prior to use. DMSO and Tris-HCl buffer were respectively purchased from Merck (Germany) and Sigma-Aldrich (USA) while Boc-Gly-Arg-Arg-MCA was purchased from Peptide Institute, Inc (Japan).
Plant Material M. cinnamomea was collected from Johor in 2003. The plant was identified by Mr. Teo Leong Eng and a voucher specimen (KL 5043) has been deposited with the University of Malaya herbarium.
Plant Material M. cinnamomea was collected from Johor in 2003. The plant was identified by Mr. Teo Leong Eng and a voucher specimen (KL 5043) has been deposited with the University of Malaya herbarium.
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