Deepsee 690 1040 nm

Manufactured by Spectra-Physics

The DeepSee 690–1040 nm is a tunable ultrafast laser system produced by Spectra-Physics. It operates within the wavelength range of 690 to 1040 nanometers and is designed to provide high-performance ultrafast laser pulses for various applications.

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Lab products found in correlation

Fv1000, by Olympus (2 mentions) Xlslpln25xgmp, by Olympus (2 mentions) Xlpln10xsvmp, by Olympus (1 mentions)

Close spelling variants of this product

DeepSee (55 citations)

2 protocols using deepsee 690 1040 nm

Multi-Scale Fluorescent Imaging of Cleared Samples

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All images were acquired using either the confocal or two photon setting on a commercial multiphoton laser scanning inverted microscope (Olympus FV1000) equipped with filter set (460–500, 520–560, 525–625, 650–700 nm) and a mode-locked Ti:sapphire laser (Mai Tai DeepSee 690–1040 nm, Spectra-Physics). Optical cleared and stained samples were immersed in CUBIC 2 reagent in a custom-made sample holder when imaged using a 10× ScaleView objective (XLPLN10XSVMP, Olympus USA; NA = 0.6 and WD = 8 mm) or placed on a coverslip when imaged using the 25× objective (XLSLPLN25XGMP, Olympus USA; NA = 1.0 and WD = 8 mm). Use of confocal versus multiphoton was dictated by which method better excited the Alexa fluorophores, which depended on fluorophore vendor and imaging depth. When acquiring images to be stitched, MATL was used in the Olympus software to program 10% overlap between imaging stacks.

Guldner I.H., Yang L., Cowdrick K.R., Wang Q., Alvarez Barrios W.V., Zellmer V.R., Zhang Y., Host M., Liu F., Chen D.Z, & Zhang S. (2016). An Integrative Platform for Three-dimensional Quantitative Analysis of Spatially Heterogeneous Metastasis Landscapes. Scientific Reports, 6, 24201.

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Deep Tissue Imaging with Olympus and Caliber

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Deep tissue imaging was performed on either an Olympus FV1000 or a Caliber ID RS-G4. While using the Olympus FV1000, either the confocal or two photon setting was used depending on which setting optimally excited the fluorophores present in the sample. On the Olympus FV1000, BrightZ setting was applied to increase signal as deeper areas of the sample were imaged. In all cases, a 25x objective (XLSLPLN25XGMP, Olympus USA; NA = 1.0 and WD = mm) was used on the Olympus FV1000. The Olympus FV1000 was equipped with filter set (460–500, 520–560, 525–625, 650–700 nm) and a mode-locked Ti:sapphire laser (Mai Tai DeepSee 690–1040 nm, Spectra-Physics). For the Caliber ID RS-G4, all images were acquired with a 20x objective. Imaging of 2D tissue slides was performed on an Olympus BX43 upright microscope for non-fluorescent histology and a Leica DM550 B inverted microscope for fluorescent histology. Gross imaging of whole brains was captured on a Leica M165 FC fluorescence stereoscope.

Guldner I.H., Wang Q., Yang L., Golomb S.M., Zhao Z., Lopez J.A., Brunory A., Howe E.N., Zhang Y., Palakurthi B., Barron M., Gao H., Xuei X., Liu Y., Li J., Chen D.Z., Landreth G.E, & Zhang S. (2020). CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10. Cell, 183(5), 1234-1248.e25.

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