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2 protocols using secondary horse radish peroxidase conjugated antibodies against mouse and rabbit

1

Epithelial Mesenchymal Transition Protein Analysis

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E-cadherin (BD Transduction Labs, 610182; used for immunoblotting), E-cadherin (Zymed, 13-1900; used for immunofluorescence stainings), N-cadherin (Takara, M142), Zona Occludens-1 (Zymed, 617300), Paxillin (BD, 610052), Fibronectin1 (Sigma-Aldrich, F3648), Vimentin (Novus Biological, NB300–223), α-Tubulin (Sigma, T-9026), GAPDH (Abcam, ab9485), Alexa-Fluor 488 and 568 (Molecular Probes), secondary horse radish peroxidase (HRP)-conjugated antibodies against mouse and rabbit (Jackson ImmunoResearch), Phalloidin Alexa-Fluor 568 (Molecular Probes, A12380), 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, D9542), recombinant human TGFβ1 (R&D Systems, 240-B).
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2

Epithelial-Mesenchymal Transition Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
E-cadherin (BD Transduction Labs, 610182), N-cadherin (Takara, M142), ZO-1 (Zymed, 617300), paxillin (BD, 610052), fibronectin (Sigma-Aldrich, F3648), vimentin (Sigma-Aldrich, V2258), GAPDH (Sigma-Aldrich, G8795), Zeb1 (Cell Signaling, 3396 and Santa Cruz Biotechnology, sc-25388), Alexa-Fluor 488 and 568 (Molecular Probes), secondary horse radish peroxidase (HRP)-conjugated antibodies against mouse and rabbit (Jackson ImmunoResearch), recombinant human TGFβ1 (R&D Systems, 240-B), 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) and phalloidin Alexa-Fluor 568 (Molecular Probes, A12380).
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