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Ctm 651416

Manufactured by Horizon Discovery
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The CTM-651416 is a specialized laboratory equipment designed for conducting various scientific experiments and analyses. It functions as a multipurpose instrument capable of performing a range of tasks, but without further details on its specific features or intended applications.

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Lab products found in correlation

Sigenome smartpool, by Horizon Discovery (2 mentions) Effectene, by Qiagen (2 mentions) Ctm 651415, by Horizon Discovery (2 mentions)

2 protocols using ctm 651416

1

Targeted Silencing of Key Regulators

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Target-specific pools of four independent small interfering RNA (siRNA) species against human CD98hc (M-003542-02), GEF-H1 (M-009883-01), GEF-H1 3′-UTR (CTM-651415, CTM-651416), eIF4A1 (M-020178-01), eIF4A2 (M-013758-01), CPSF73 (M-006365-00), eEF1ε1 (M-015983-01), and non-targeting siRNA control pool #1 (D-001206-13) (siGENOME SMARTpool) were purchased from Dharmacon (Horizon Discovery). siRNA pools were transfected using Effectene (QIAGEN) at a final concentration of 50 nM for 48 hr unless otherwise stated before subsequent treatments. ARHGEF2/GEF-H1 ORF cDNA construct (OHu26696) and empty vector were purchased from GenScript, and transfected using Effectene (QIAGEN) following manufacturer protocols.
Ho J.J., Cunningham T.A., Manara P., Coughlin C.A., Arumov A., Roberts E.R., Osteen A., Kumar P., Bilbao D., Krieger J.R., Lee S, & Schatz J.H. (2021). Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. Cell Reports, 37(2), 109806.
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2

Targeted siRNA-mediated knockdown

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Target-specific pools of four independent small interfering RNA (siRNA) species against human CD98hc (M-003542-02), GEF-H1 (M-009883-01), GEF-H1 3′-UTR (CTM-651415, CTM-651416), eIF4A1 (M-020178-01), eIF4A2 (M-013758-01), CPSF73 (M-006365-00), eEF1ε1 (M-015983-01), and non-targeting siRNA control pool #1 (D-001206-13) (siGENOME SMARTpool) were purchased from Dharmacon (Horizon Discovery). siRNA pools were transfected using Effectene (QIAGEN) at a final concentration of 50 nM for 48 hr unless otherwise stated before subsequent treatments. ARHGEF2/GEF-H1 ORF cDNA construct (OHu26696) and empty vector were purchased from GenScript, and transfected using Effectene (QIAGEN) following manufacturer protocols.
Ho J.J., Cunningham T.A., Manara P., Coughlin C.A., Arumov A., Roberts E.R., Osteen A., Kumar P., Bilbao D., Krieger J.R., Lee S, & Schatz J.H. (2021). Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. Cell Reports, 37(2), 109806.
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