Poloxamer 188 p188 lutrol f68

All materials used were of reagent grade and from commercial sources. The MNPs (iNANOvative™) were kindly provided by Nanos Scientificae Ltd. (Nanos SCI, Ljubljana, Slovenia). The MNPs used were composed of self-assembled multiple 10 nm superparamagnetic iron oxide nanocrystals and these nanocrystals clusters were then coated with a 10 nm thick silica shell. All procedures of MNP synthesis were described in our published studies (Kralj and Makovec, 2015; (link)Nemec et al., 2020; (link)Nemec and Kralj, 2021; (link)Tadic et al., 2014) (link). These MNPs were used in the form of ethanol-or water-based suspensions. Polyethylene oxide (PEO; Mw 400,000 g/mol) was from Sigma-Aldrich, Co. (St. Louis, USA), poloxamer 188 (P188; Lutrol F68) from BASF (Ludwigshafen, Germany), sodium chloride from Merck KGaA (Darmstadt, Germany), and ethanol (96 %, v/v) from Pharmachem Sušnik Jožef (Ljubljana, Slovenia). The water used was purified by reverse osmosis.

All materials used were of reagent grade and from commercial sources. Ibuprofen was from Fagron Hellas (Trikala, Greece), carvedilol was a gift from Krka d.d. (Novo mesto, Slovenia), paracetamol was a gift from Lek d.d. (Ljubljana, Slovenia), and metformin hydrochloride was a gift from Zentiva k.s. (Prague, Czech Republic). Polyethylene oxide (PEO; Mw, 400,000 g/mol) was from Sigma-Aldrich, Co. (St. Louis, MO, USA) and poloxamer 188 (P188; Lutrol ® F68) from BASF (Ludwigshafen, Germany). Hydrochloric acid (37%, w/w; HCl), orthophosphoric acid (85%, w/w; H 3 PO 4 ), sodium hydroxide (NaOH), potassium dihydrogen phosphate (KH 2 PO 4 ), formic acid (98-100%, w/w, HCOOH), and polysorbate 80 (Tween ® 80) were from Merck KGaA (Darmstadt, Germany). Acetonitrile was from J.T. Baker (Gliwice, Poland), and ethanol (96%, v/v) was from Pharmachem Sušnik Jožef (Ljubljana, Slovenia). The water used was purified by reverse osmosis and Milli-Q water was obtained by the Millipore Milli-Q lab water system.