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Custom made single stranded dna oligomers

Manufactured by Merck Group

Custom made single stranded DNA oligomers are short, synthetic DNA sequences that can be tailored to specific requirements. They serve as essential components in a variety of molecular biology and genetic research applications.

Automatically generated - may contain errors

2 protocols using custom made single stranded dna oligomers

1

BPDE-DNA Adduct Formation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Benzo[a]pyrene-r-7,t-8-dihydrodiol-t-9,10-epoxide (+/-) (anti) (anti BPDE) was from National Cancer Institute Chemical Carcinogen Reference Standard Repository. Triethylammonium bicarbonate (TEAB, 1.0 M, pH 8.4-8.6) and 4-nitrobenzyl alcohol were from Sigma-Aldrich. Custom made single stranded DNA oligomers and the 32-base pair oligonucleotide were from Sigma-Aldrich. HPLC grade solvents, methanol, tetrahydrofuran and water were from Fischer Scientific. Restriction enzyme, NIaIII was from New England Biolabs.
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2

DNA Adduct Identification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Benzo[a]pyrene-r-7,t-8-dihydrodiol-t-9,10-epoxide (±) (anti) (anti BPDE) was from National Cancer Institute Chemical Carcinogen Reference Standard Repository. Triethylammonium bicarbonate (TEAB, 1.0 M, pH 8.4–8.6) and 4-nitrobenzyl alcohol were from Sigma-Aldrich. Custom made single stranded DNA oligomers and the 32-base pair oligonucleotide were from Sigma-Aldrich. HPLC grade solvents, methanol, tetrahydrofuran and water were from Fischer Scientific. Restriction enzyme, NlaIII was from New England Biolabs.
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