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Precellys cryolys

Manufactured by Bertin Technologies
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Sourced in France

The Precellys Cryolys is a high-performance homogenizer designed for efficient sample preparation. It utilizes cryogenic cooling to maintain sample integrity during the homogenization process.

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Lab products found in correlation

Dneasy kit, by Qiagen (1 mentions) Certiprep, by SPEX (1 mentions)

Spelling variants of this product

Cryolys (11 citations) Precellys Cryolys Evolution (5 citations) Cryolys Precellys 24 sample Homogenizer (5 citations) Cryolys Precellys 24 (3 citations)

3 protocols using precellys cryolys

1

Quantifying Splenic Cytokine Profiles

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Under 5% isoflurane anaesthesia, spleens were dissected and snap frozen in liquid nitrogen for storage at -80°C. Spleens were thawed on ice and weighed, protein was extracted from ~20mg of spleen tissue per 100μl of protein extraction lysis buffer. Tissue was homogenised using a Precellys-Cryolys (Bertin Instruments, Montigny-le-Bretonneux, France). Lysate was then centrifuged, and supernatant stored at -80°C prior to analysis. The Bio-plex 23 cytokine/chemokine mouse array bead kit was used for cytokine/chemokine quantification following the manufacturer’s instructions (Bio-Rad, Hercules, CA) using protein from 4mg of spleen tissue per mouse. The beads were analysed using a MAGPIX system (Luminex, Austin, TX) and the medium fluorescent intensity values (corrected for background reading) were compared.
Lees J.G., White D., Keating B.A., Barkl-Luke M.E., Makker P.G., Goldstein D, & Moalem-Taylor G. (2020). Oxaliplatin-induced haematological toxicity and splenomegaly in mice. PLoS ONE, 15(9), e0238164.
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2

Cartilage Extraction and DNA Isolation

Cited 1 time
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Full-thickness cartilage sections were dissected from the femoral head (human hip samples) or tibial plateau (human and mouse knee samples). A representative section was saved in 4% paraformaldehyde for histopathological scoring. Approximately 200mg of cartilage tissue was cryogenically ground using a grinder mill (Spex CertiPrep, Middlesex, UK), murine samples were cryogenically ground using a Precellys Cryolys (Bertin, Bretonneux, France). DNA was isolated using a DNeasy kit (Qiagen). Plasticware and reagents were decontaminated by a 30-minute UV exposure as previously described(21 (link),22 (link)). PCR master mixes were decontaminated with double-stranded DNAse treatment (PCR decontamination kit, Arcticzymes, Tromsø, Norway). Sterile water was processed using the same (murine) procedure as a negative control.
Dunn C.M., Velasco C., Rivas A., Andrews M., Garman C., Jacob P.B, & Jeffries M.A. (2020). Identification of cartilage microbial DNA signatures and associations with knee and hip osteoarthritis. Arthritis & rheumatology (Hoboken, N.J.), 72(7), 1111-1122.
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3

Metabolite Extraction from Liver and Feces

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The frozen liver and lyophilized fecal samples were ground with liquid nitrogen using mortar and pestle, pre-weighed in the lysis tubes, and homogenized in tissue homogenizer (Precellys Cryolys, Bertin Technologies SAS) by the addition of MeOH:H2O (2:1) with 0.1% FA (1500 μL) and ceramic beads (for 2 × 20 seconds at 10,000 rpm). Homogenized extracts were centrifuged for 15 minutes at 21,000 g at 4°C, and the aliquots (50 μL) of supernatants were pre-mixed with 100 μL of the ice-cold IS mixture (in 100% MeOH), and 600 μL of H2O with 0.2% formic acid, and loaded onto solid-phase extraction (SPE) plates for further processing as previously described (Sorrentino et al., 2020 (link)). Plasma aliquots (25 μL) were prepared in the same way (with the addition of the IS mixture) and processed using SPE for the phospholipid removal and BA pre-concentration.
Li H., Perino A., Huang Q., Von Alvensleben G.V., Banaei-Esfahani A., Velazquez-Villegas L.A., Gariani K., Korbelius M., Bou Sleiman M., Imbach J., Sun Y., Li X., Bachmann A., Goeminne L.J., Gallart-Ayala H., Williams E.G., Ivanisevic J., Auwerx J, & Schoonjans K. (2022). Integrative systems analysis identifies genetic and dietary modulators of bile acid homeostasis. Cell Metabolism, 34(10), 1594-1610.e4.
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