Pdyn cre

To label genetically distinct subsets of SOM interneurons, we crossed Sst-Flp mice (catalog #028579, The Jackson Laboratory; He et al., 2016 (link)) with one of the following four Cre recombinase-expressing mouse lines: Calb2-Cre (strain #010774, The Jackson Laboratory; Taniguchi et al., 2011 (link)), Calb1-Cre (strain #028532, The Jackson Laboratory; Daigle et al., 2018 (link)), Chrna2-Cre (Leão et al., 2012 (link)), and Pdyn-Cre (strain #027958, The Jackson Laboratory; Krashes et al., 2014 (link)). Dual-recombinase progeny were then crossed with the RC::FLTG reporter line (strain #026932, The Jackson Laboratory; Plummer et al., 2015 (link)) to create triple-transgenic mice expressing GFP in Cre⁺/Flp⁺ cells and tdTomato in Cre^–/Flp⁺ cells. We also used X94 and X98 mice (strains #006340 and #006334, The Jackson Laboratory; Ma et al., 2006 (link)) to label previously characterized subsets of L4-projecting and L1-projecting SOM cells, respectively, crossing them with Cre driver lines and the Ai9 tdTomato reporter (strain #007909, The Jackson Laboratory; Madisen et al., 2010 (link)).

Animal experiments were approved by The Rockefeller University Animal Care and Use Committee and were carried out in accordance with the National Institutes of Health guidelines. All experiments were conducted on male mice older than 8 weeks of age housed in a 12-h light/12-h dark cycle at 22 °C and 30–60% humidity. We used the following genotypes of mice: C57BL/6J (wild type; number 000664, The Jackson Laboratory), FOS^2A-iCreER (TRAP2) mice (The Jackson Laboratory, stock number 030323), CAG–Sun1/sfGFP (INTACT) mice (The Jackson Laboratory, stock number 021039), VGLUT2–IRES–Cre mice (The Jackson Laboratory, stock no. 028863), VGAT–IRES–Cre mice (The Jackson Laboratory, stock no. 028862), ADCYAP1–2A–Cre mice (The Jackson Laboratory, stock number 030155), PHOX2B–Cre (The Jackson Laboratory, Stock 016223), DBH–Cre (The Jackson Laboratory, stock number 033951), TH–Cre (The Jackson Laboratory, stock number 008601), TAC1–Cre (The Jackson Laboratory, stock number 021877), PDYN–Cre (The Jackson Laboratory, stock number 027958), CCK–Cre (The Jackson Laboratory, stock number 012706), NTS–Cre (The Jackson Laboratory, stock number 017525). For all Cre mouse line experiments, only heterozygous animals were used. Sample sizes were decided based on experiments from similar studies. Animals were randomly assigned to groups when possible.