Fluorescein isothiocyanate fitc conjugated f4 80

The antibodies for CARD9, CD206, CD68, TGF-β, IL-13, IL-4, IL-18, BCL10, MALT1, GAPDH, β-actin and IgG were from Santa Cruz Biotechnology (Santa Cruz, CA, USA), the phospho-Syk antibody sampler kit, NF-κB pathway sampler kit were from Cell Signaling Technology (Beverly, MA, USA); the antibody for F4/80 was from Abcam (Cambridge, MA, USA); and ChemMate TM EnVision System/DAB Detection Kits were from Dako (Glostrup, Denmark). Antibodies for PerCP/Cy5.5-conjugated CD45.2, phycoerythrin (PE)-conjugated F4/80, fluorescein isothiocyanate (FITC)-conjugated F4/80, FITC-conjugated CD206, PE-conjugated CD3, FITC-conjugated CD4, FITC-conjugated CD8 and isotype control were from Biolegend (San Diego, CA, USA). FITC-conjugated IL-10, FITC-conjugated IL-1α and PE-conjugated IL-12 were from eBioscience (San Diego, CA, USA). Piceatannol and BAY61-3606 were from Sigma (St. Louis, MO, USA). Bio-Plex Cytokine Assay Kits were from Bio-Rad Laboratories (Hercules, CA, USA). Neutralizing anti-VEGF Ab and anti-MIG Ab were from R&D Systems (Minneapolis, MN, USA). Neutralizing anti-IL-15 Ab was from Abcam.