Synergy2 cam4

Example 3

Aminoglycosides were screened for polymerization reaction using optical density measurements. Six aminoglycosides were screened, namely, gentamicin sulfate (Sigma, Cat #G1914), neomycin sulfate (Sigma, Cat #PHR1491), streptomycin sulfate (Sigma, Cat #S6501), tobramycin sulfate (Sigma, Cat #T1783), kanamycin sulfate (Sigma, Cat #60615), and kanamycin disulfate (Sigma, Cat #K1876). 100 μl of 10 milligram per milliliter (mg/ml) aqueous solutions of antibiotics, 100 μl of deionized (DI) water, and either 100 μl of algae extract or 100 μl of 10 mg/ml sodium alginate (Sigma, Cat #W201502) were mixed together to obtain a total reaction volume of 300 μl in each well of 96-well microtiter plates. To vary the concentration of antibiotics, the subsequent wells were filled with 80 μl, 60 μl, 40 μl, 20 μl, and 10 μl of 10 mg/ml antibiotic stock solution with the remaining volume out of 300 μl reactions compensated by deionized (DI) water. Optical density at 600 nanometers (nm) (OD600) at room temperature was measured using a plate reader (BioTek, Model #Synergy2-Cam4, Software-Gen5-1.08). The results are shown in FIG. 1A, discussed further below. A Nikon Coolpix camera was used to image wells in microtiter plates, and the results are shown in FIG. 1B, discussed further below.