This study was approved by the Animal Experiment Committee of Kagoshima University and was conducted according to the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals. All efforts were made to minimize the number of animals included. We used 79 male C57BL/6J mice weighing 20–30 g (Japan SLC, Shizuoka, Japan) and C57BL/6-Tg (UBC-GFP) 30Scha/J (green-fluorescent-protein-transgenic; GFP-Tg) mice (Jackson Laboratory, Bar Harbor, ME, USA). Surgery was performed under anesthesia induced by an intraperitoneal injection of 0.3 mg/kg medetomidine (Meiji Seika Pharma, Tokyo, Japan), 4.0 mg/kg midazolam (Novartis, Basel, Switzerland), and 5.0 mg/kg butorphanol (Meiji Seika Pharma).
Gfp tg mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
GFP-Tg) mice are a transgenic mouse model that expresses the green fluorescent protein (GFP) gene. These mice are used as a research tool to study various biological processes and cell populations by exploiting the fluorescent properties of GFP.
Automatically generated - may contain errors
Lab products found in correlation
Midazolam, by Novartis (2 mentions)
C57bl 6 tg ubc gfp 30scha j green, by Jackson ImmunoResearch (2 mentions)
Medetomidine, by Meiji Seika Pharma (2 mentions)
C57bl 6j mice, by Japan SLC (2 mentions)
Butorphanol, by Meiji Seika Pharma (2 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Histopaque 1083, by Merck Group (1 mentions)
3 protocols using gfp tg mice
1
Mice Anesthesia and Surgical Protocol
2
Isolation and Culture of Mouse BM-Derived Cells
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Green fluorescent protein-transgenic (GFP-Tg) mice from the C57BL/6J background were obtained from the Jackson Laboratory (Bar Harbor, ME). All animal procedures in this study were approved by the institutional animal care and use committee of Samsung Medical Center, Seoul, Republic of Korea. Male GFP-Tg and wild-type C57BL/6J mice aged 10 to 12 weeks were used for obtaining bone marrow (BM) from femurs and tibias. BM was flushed by injection of phosphate-buffered saline (PBS), dispersed by pipetting, and isolated by density gradient centrifugation with Histopaque-1083 (Sigma, St Louis, MO). Mononuclear cells (MNCs) obtained from BM were cultured as described previously with minor modifications (14) and isolated from GFP-Tg mice to trace in vivo transplanted cells. Briefly, 3-5 Â 10 6 MNCs/mL were suspended on HydroCell TM ultra-low attach surface (Nunc) with endothelial basal medium-2 (EBM-2) supplemented with EGM-2 SingleQuots (EGM-2 medium, Lonza Inc, Walkersville, MD) and 5% fetal bovine serum (FBS) at 378C in a 5% CO 2 humidified incubator. After 2 days of culture, fresh media was added without changing the media.
3
Anesthesia for Mouse Surgery
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This study was approved by the Animal Experiment Committee of Kagoshima University and was conducted according to the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals. All efforts were made to minimize the number of animals included. We used 77 male C57BL/6J mice weighing 20-30 g (Japan SLC, Shizuoka, Japan) and C57BL/6-Tg (UBC-GFP) 30Scha/J (green-uorescent-protein-transgenic; GFP-Tg) mice (Jackson Laboratory, Bar Harbor, ME, USA). Surgery was performed under anesthesia induced by an intraperitoneal injection of 0.3 mg/kg medetomidine (Meiji Seika Pharma, Tokyo, Japan), 4.0 mg/kg midazolam (Novartis, Basel, Switzerland), and 5.0 mg/kg butorphanol (Meiji Seika Pharma).
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