Mouse fibroblasts were plated on standard tissue culture 6-well plastic plates (BD Falcon Labware). Images were acquired with a Nikon BioStation CT (Nikon) equipped with GFP and mCherry fluorescence filters and phase contrast using 2× lenses. The single-plane images of each channel were stitched automatically using the automated image analysis software CL-Quant 3.0 (Nikon).
Cl quant 3
Manufactured by Nikon
The CL-Quant 3.0 is a laboratory instrument designed for the quantitative analysis of cell samples. It provides automated cell counting and viability assessment capabilities.
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Lab products found in correlation
2 protocols using cl quant 3
1
Live-cell fluorescent imaging of fibroblasts
2
Fluorescence Imaging of Mouse Fibroblasts
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Mouse fibroblasts were plated on standard tissue culture 6-well plastic plates (Greiner, cat. no. 657160). Images were acquired with a Nikon BioStation CT (Nikon) equipped with GFP and mCherry fluorescence filters and phase contrast using 2× lenses. The single-plane images of each channel were stitched automatically using the automated image analysis software CL-Quant 3.0 (Nikon).
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