Gefitinib sml1657

Salt oleic acid (sodium oleate; sc-215879) was purchased from Santa Cruz (Santa Cruz). Linoleic acid sodium salt (L8134), sodium palmitate (P9767), N-acetylcysteine (NAC; A1965), U0126 (19-147), gefitinib (SML1657), and erlotinib (SML2156) were purchased from Sigma‒Aldrich (Sigma‒Aldrich, St Louis, MO, USA). Bovine serum albumin (fatty acid-free; A7030) (Sigma‒Aldrich, St Louis, MO, USA) was conjugated with oleic acid at a 1:8 molar ratio (BSA/OA). Carboxyl H2-DCFDA (D399) and EGF (PHG0311) were purchased from Invitrogen (Invitrogen, Grand Island, NY, USA). Recombinant human ANGPTL4 (rh-ANGPTL4: 4487-AN) was purchased from R&D Systems (R&D Systems, Minneapolis, MN). CXCL8 (ab9631) was purchased from Abcam (Abcam). Cetuximab was purchased from BioVision (A1047-100; BioVision). Vitamin E (25985), hygromycin B (hydrate: 14291), reparixin (21492), and atorvastatin (10493) were purchased from Cayman (Cayman). atorvastatin (Lipitor; VITARIS) was used for feeding mice.

The antibodies were procured from Cell Signaling Technology (Danvers, MA, USA), BioLegend (San Diego, CA, USA), Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), BD Biosciences (San Jose, CA, USA), and GeneTex (Irvine, CA, USA, Table S1). Metformin (D150959) and propidium iodide (PI, 81845), Hoechst 33342 (H3570), rapamycin (553210), SB202190 (S7067), and Gefitinib (SML1657) were purchased from Sigma-Aldrich (Burlington, MA, USA) and Thermo Fisher Scientific (Waltham, MA, USA), respectively. Fluor^TM 594 phalloidin (RCS2314) and MitoTracker Green fluorescent dye (RMS1101) for mitochondrial visualization were purchased from BioActs (Inchon, Korea).

Cells were seeded into 96-well plates at a density of 2 × 10³ cells/well and incubated with 5% CO₂ at 37 °C for cell adherence. Each well was added with 10 uL of the CCK-8 reagent, and then, the optical density (OD) value was measured at a wavelength of 450 nm at variable time points of 24, 48, 72, and 96 h.
Then, the cell resistance was evaluated following procedures from existing research [21 (link)]. A total of 5 × 10³ cells were seeded into the 96-well plates and incubated for 24 h after transfection. Next, the cells were further incubated in variable concentrations of gefitinib (SML1657, Sigma-Aldrich Chemical Company, St Louis, MO, USA) or cisplatin (P4394, dissolved in 0.15 M NaCl, Sigma-Aldrich Chemical Company, St Louis, MO, USA) for 48 h. The cells were incubated with the CCK-8 reagent at 37 °C for 4 h. The OD value was measured at a wavelength of 570 nm using a Microplate Reader (Sunrise Microplate Reader, TECAN, Switzerland).