LV pieces were thawed, stretched, fixed, frozen, cryosectioned, and stained as previously described (15 (link)). Primary antibodies included rabbit polyclonal anti-Tmod1 (2 μg/ml) and mouse monoclonal anti–α-actinin (1:200; EA-53, Sigma-Aldrich) antibodies. Secondary antibodies included Alexa Fluor 488–conjugated donkey anti-rabbit IgG (1:1000) and Alexa Fluor 350–conjugated goat anti-mouse IgG (1:200). Texas Red–conjugated phalloidin (1:50) was used to stain F-actin (Thermo Fisher Scientific). Images were captured using a DeltaVision RT system (GE Healthcare) with a 100× numerical aperture 1.3 objective and a charge-coupled device camera (CoolSNAP HQ, Photometrics). Images were deconvolved using SoftWoRx software and processed using Photoshop CC (Adobe). Thin filament lengths were measured by Tmod1 staining using the DDecon plugin for ImageJ (30 (link)).
Deltavision rt system
Manufactured by GE Healthcare
Sourced in United States
The DeltaVision RT system is a high-resolution imaging system designed for advanced fluorescence microscopy. It offers a modular and customizable platform for live-cell and fixed-sample imaging.
Automatically generated - may contain errors
2 protocols using deltavision rt system
1
Thin Filament Length Measurement in LV Tissue
2
Imaging and Measuring Thin Filament Lengths
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 16–21 images (per group) of LV sections with visible Tmod1 doublet staining at thin filament pointed ends were taken using a Deltavision RT system (GE Healthcare, Chicago, IL, USA) with a 100× NA 1.3 objective and a CCD camera (CoolSNAP HQ; Teledyne Photometrics, Tucson, AZ, USA), deconvolved using SoftWoRx software and processed using ImageJ (National Institutes of Health, Bethesda, MD, USA). Thin filament lengths (at least 4 thin filaments per image) were measured by Tmod1 staining using the ImageJ plugin DDecon [20 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!