Anti pdpn

Cell Signaling Technologies (CST) anti-CD133, CD146, CDH2, CLDN1, ERK were purchased from Ozyme (Saint-Quentin-en-Yvelines, FRANCE). Anti-p-ERK and anti-PDPN were purchased from Abcam (Paris, FRANCE), as well as goat anti-rabbit Alexa 488 and goat anti-mouse Alexa 594 IgGs. Invitrogen anti-α-tubulin was purchased from Thermo Fisher. Anti-ARD1 was a homemade polyclonal antibody made against the last 20 C-terminal amino acids of the human ARD1 sequence, as described earlier^{63 (link)}. HRP-coupled anti-rabbit and anti-mouse IgG (Promega, Charbonnières-les-Bains, FRANCE) were used (1:5000) as secondary antibodies for immunoblotting experiments.
A list of all the antibodies used in this study is provided in Supplementary Table S3.

Five-micron sections of tissue were cut by cryosectioning and stained with conjugated or purified antibodies. Purified antibodies were detected using secondary antibodies. The antibodies used were FITC anti-CD41 (clone MWReg30, BioLegend), or APC anti-CD41 (clone, MWReg30, BioLegend), anti-Lyve1 (rabbit polyclonal; Abcam), anti-PROX1 (rabbit polyclonal, clone Poly19252, BioLegend), FITC anti-CD61 (#M031-1, Emfret), FITC anti-CD42b, (#M040-1, Emfret), FITC anti-CD62P (clone CLB-Thromb/6, Beckman), anti-GPIbβ (CD42c, #M050-0, Emfret), MECA79 (Santa Cruz, sc-19602), anti-PDPN (Abcam, ab11936), anti-Mast Cell Chymase (Proteintech, 18189-I-AP). The sections were visualized using the Evos FL Auto 2 microscope from Invitrogen (Thermo Fisher Scientific) for whole images and Nikon confocal microscope for high-resolution images.