A0502

Manufactured by Beyotime

Sourced in China

The A0502 is a laboratory equipment used for various applications in scientific research and analysis. It is designed to perform specific functions, but the details of its core function and intended use are not available without the risk of extrapolation or interpretation.

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Lab products found in correlation

4 protocols using a0502

Quantitative Analysis of Pancreatic Islet Markers

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PCNA and CD68 were detected by immunohistochemistry (IHC), while insulin and glucagon were revealed by immunofluorescence staining with a previously described procedure^{28 (link),29 (link)}. Sections were incubated with the primary antibody, mouse anti-PCNA (AF0261, Beyotime, China), rabbit anti-CD68 (BA3638, Boster, China), goat anti-insulin (sc-7839, Santa Cruz, Santa Cruz, CA), or mouse anti-glucagon (BM1621, Boster, China) at 4 °C overnight, and with a secondary antibody, HRP-goat anti-mouse (Catalog#: 32230, Zymed, San Francisco, CA), HRP-goat anti-rabbit (A0208, Beyotime, China), donkey anti-goat cy3 (A0502, Beyotime, China), or goat anti-mouse FITC (A0568, Beyotime, China) for 1 h at room temperature. For IF, the nucleus was stained with DAPI (1 μg/ml, Dojindo, Japan) after washing with PBS. A total of 3 randomly chosen pancreatic sections from each mouse in each group (about 40 islets) were used for IHC or IF study. Finally, images were collected at equal exposure conditions and at the same magnification (20x objective lens) and analyzed by using the Image-Pro Plus 6.0 software.

Wang N., Tian X., Chen Y., Tan H.Q., Xie P.J., Chen S.J., Fu Y.C., Chen Y.X., Xu W.C, & Wei C.J. (2017). Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice. Scientific Reports, 7, 14707.

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Quantification of Chondrocyte Anabolic Markers

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The control ADSCs, GFP-ADSCs and IGF-1-ADSCs were harvested 16 h after transfection. The expression of IGF-1 (Abcam, ab106836, dilution: 1:50) was detected. The chondrocytes were harvested 48 h after treatment. Immunofluorescence was used to evaluate the anabolic markers of the chondrocytes. The anabolic markers including Col2(Santa Cruz, sc-52658, dilution: 1:200) and Aggrecan (Abcam, ab36861, dilution: 1:200) were detected. Cells were firstly fixed in 4% (vol/vol) paraformaldehyde for 30 min and subjected to immunofluorescence for primary anti-bodies at 4 °C overnight. After washed by PBS, cells were incubated with secondary anti-bodies (Alexa Fluor® 488, A21202 for Col2, dilution: 1:500; Alexa Fluor® 546, A11035 for Aggrecan, dilution: 1:500; Cy3, Beyotime Institute of Biotechnology, A0502 for IGF-1, dilution: 1:500) and the nucleus was visualized with DAPI. The sections were viewed under a Nikon A1R confocal laser scanning microscope (Nikon, Tokyo, Japan).

Wu H., Peng Z., Xu Y., Sheng Z., Liu Y., Liao Y., Wang Y., Wen Y., Yi J., Xie C., Chen X., Hu J., Yan B., Wang H., Yao X., Fu W, & Ouyang H. (2022). Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development. Stem Cell Research & Therapy, 13, 19.

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Immunohistochemistry Protocol for Antigen Detection

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The sections were routinely deparaffinized and hydrated, and then the sections were heated to 95°C in Tris-EDTA buffer (pH 9.0) for 15 min for antigen retrieval. Subsequently, the sections were incubated with 1% bovine serum albumin (BSA) in phosphate- buffered saline (PBS) for 30 min at 37°C to block nonspecific sites. Third, the sections were incubated respectively with different primary antibodies (data on the primary antibodies are described in Table 1) at 4°C overnight, followed by incubation with Cy3-labeled goat anti-rabbit IgG (H + L) (A0516, Beyotime, Jiangsu, China) for rabbit-derived primary antibodies, Cy3-labeled goat anti-mouse IgG (H+L) (A0521, Beyotime) for mouse-derived primary antibodies, or Cy3-labeled donkey anti-goat IgG (H+L) (A0502, Beyotime) for goat-derived primary antibody, at room temperature in the dark at 1:500 dilution for 1 h. Finally, the sections were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI; Beyotime) for 2 min at room temperature in the dark and mounted with anti-fade mounting medium (P0128, Beyotime). PBS was used for rinsing between steps. Sections omitting the primary antibodies or using normal serum of the same species as the primary antibodies were used as negative controls.

Cao M., Zhang L., Cheng J., Wang C., Zhao J., Liu X., Yan Y., Tang Y., Chen Z, & Li H. (2022). Differential antigen expression between human apocrine sweat glands and eccrine sweat glands. European Journal of Histochemistry : EJH, 67(1), 3559.

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Immunofluorescence Staining of LUAD Cells

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The constructed plasmid or indicated siRNAs were transfected into LUAD cells for 24h. LUAD Cells were plated on coverslips for 24h. After fixed with 4% paraformaldehyde for 20 min at room temperature, Cells were permeabilizated using 0.1% Triton X-100 for 7 min. Next, after blocked with 2% BSA in PBS for 2h, Cells were incubated with primary antibody overnight at 4 °C (1:500 for SAM68 and hnRNP A1). After 3 times wash with 0.1% PBST, secondary goat anti-rabbit cy3 (beyotime, A0428, 1:500 dilution) or goat anti-mouse AlexaFluor 488 (beyotime, A0502, 1:500 dilution) were added for 2 h incubation. After 3 times wash with 0.1% PBST, coverslips were stained with DAPI (beyotime, C1005).

Zhu S., Chen W., Wang J., Qi L., Pan H., Feng Z, & Tian D. (2021). SAM68 promotes tumorigenesis in lung adenocarcinoma by regulating metabolic conversion via PKM alternative splicing. Theranostics, 11(7), 3359-3375.

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