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Pcdna6 myc ulk1 wt

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PCDNA6-myc ULK1 wt is a plasmid construct that expresses the wild-type form of the ULK1 gene under the control of a cytomegalovirus (CMV) promoter. ULK1 is a serine/threonine protein kinase that plays a key role in the initiation of autophagy, a cellular process involved in the degradation and recycling of damaged organelles and proteins.

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Lab products found in correlation

Pbabe puro mcherry gfp lc3, by Addgene (2 mentions) Pmxs purogfp dfcp1, by Addgene (2 mentions) Pcdna3 myc ampkalpha 2, by Addgene (2 mentions) Pcmv ampk alpha2 flag, by Sino Biological (2 mentions) Recombinant hipmk, by OriGene (2 mentions) Pbabepuro gfp lc3, by Addgene (2 mentions) Pcdna3 flag ulk1, by Addgene (2 mentions) Recombinant hulk1 and ampk, by Sino Biological (2 mentions)

2 protocols using pcdna6 myc ulk1 wt

1

Plasmid Toolkit for Autophagy Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following plasmids were from Addgene:
pMXs-puroGFP-DFCP1,pCDNA6-myc ULK1 wt, pCDNA3 Flag ULK1, pCDNA3 myc AMPK
Alpha 2, pBABE-puro-mCherry-GFP-LC3,and pBABE-puro-GFP-LC3. pCMV-AMPK alpha
2 Flag was from Sinobiologicals. Our lab generated pCMV-IPMK-GST and
different fragments tagged with GST, pCMV-myc-IPMK, pCMV-HA-IPMK, pMX-myc,
pMX-myc IPMK WT, and pMX-myc KSA. Constitutively active AMPK was a kind gift
from Dr. Anne Burnet (Stanford University School of Medicine, Stanford, CA).
We purchased recombinant hIPMK from Origene. Recombinant hULK1 and AMPK were
purchased from Signalchem.
Guha P., Tyagi R., Chowdhury S., Reilly L., Fu C., Xu R., Resnick A.C, & Snyder S.H. (2019). IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. Cell reports, 26(10), 2692-2703.e7.
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2

Plasmid Toolkit for Autophagy Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following plasmids were from Addgene:
pMXs-puroGFP-DFCP1,pCDNA6-myc ULK1 wt, pCDNA3 Flag ULK1, pCDNA3 myc AMPK
Alpha 2, pBABE-puro-mCherry-GFP-LC3,and pBABE-puro-GFP-LC3. pCMV-AMPK alpha
2 Flag was from Sinobiologicals. Our lab generated pCMV-IPMK-GST and
different fragments tagged with GST, pCMV-myc-IPMK, pCMV-HA-IPMK, pMX-myc,
pMX-myc IPMK WT, and pMX-myc KSA. Constitutively active AMPK was a kind gift
from Dr. Anne Burnet (Stanford University School of Medicine, Stanford, CA).
We purchased recombinant hIPMK from Origene. Recombinant hULK1 and AMPK were
purchased from Signalchem.
Guha P., Tyagi R., Chowdhury S., Reilly L., Fu C., Xu R., Resnick A.C, & Snyder S.H. (2019). IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration. Cell reports, 26(10), 2692-2703.e7.
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