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Htgf β1

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HTGF-β1 is a recombinant human Transforming Growth Factor beta-1 protein. It is a cytokine that plays a crucial role in cell growth, differentiation, and immune system regulation.

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Lab products found in correlation

Mil 6, by Miltenyi Biotec (3 mentions) Mil 23, by R&D Systems (2 mentions) Anti cd3 145 2c11, by BioXCell (1 mentions) Anti il 4 11b11, by BioXCell (1 mentions) Anti ifn γ xmg1, by BioXCell (1 mentions) Il 23, by R&D Systems (1 mentions)

3 protocols using htgf β1

1

Induction of Passive-Transfer EAE in Mice

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Naïve CD4+ T cells (CD4+Vβ11+CD62Lhi) were sorted from the spleens and lymph nodes of 2D2 TCR transgenic mice and polarized towards Th17 lineage in the presence of irradiated splenocytes (5:1 ratio) and 2.5 μg/ml anti-CD3 (145-2C11, BioXCell), 20 μg/ml anti-IL-4 (11B11, BioXCell), 20 μg/ml anti-IFNγ (XMG1.2, BioXCell), 30 ng/ml mIL-6 and 3 ng/ml hTGF-β1 (Miltenyi Biotec). The following day, cells were treated with either DMSO or FGIN-1-27 (10 μM) and IL-23 (10 ng/ml; R&D Systems) was added after 60 h of activation. On day 5 of culture, cells were reactivated on plates precoated with 2 μg/ml of anti-CD3 and anti-CD28 (PV1, BioXCell) for 48 h, and 5 million cells were adoptively transferred to recipient mice to induce passive-transfer EAE. Classical EAE symptoms were scored daily according to standard criteria: 0, asymptomatic; 1, flaccid tail; 2, hind-limb weakness and impaired righting ability; 3, hind-limb paralysis; 4, front- and hind-limb paralysis; 5, moribund or death.
Singh A., Dashnyam M., Chim B., Escobar T.M., Dulcey A.E., Hu X., Wilson K.M., Koganti P.P., Spinner C.A., Xu X., Jadhav A., Southall N., Marugan J., Selvaraj V., Lazarevic V., Muljo S.A, & Ferrer M. (2020). Anxiolytic Drug FGIN-1-27 Ameliorates Autoimmunity by Metabolic Reprogramming of Pathogenic Th17 Cells. Scientific Reports, 10, 3766.
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2

Naïve 2D2 TCR-transgenic CD4+ T Cell Differentiation

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Naïve 2D2 TCR-transgenic CD4+ T cells from spleen and lymph nodes of 2D2 wild-type or 2D2 Tbx21−/− mice were electronically sorted (CD4+Vβ11+CD62Lhi), and activated under TH17 polarizing conditions with the following: anti-CD3 (2.5 μg/ml) (145-2C11, BioXCell), anti-IL-4 (20 μg/ml) (11B11, BioXCell), anti-IFN-γ (20 μg/ml) (XMG1.2, BioXCell), mIL-6 (30 ng/ml) (Miltenyi Biotec), hTGF-β1 (3 ng/ml) (Miltenyi Biotec) in the presence of irradiated wild-type splenocytes at 5:1 ratio. After 60 h of activation, mIL-23 (10 ng/ml; R&D Systems) was added. On day 5 of culture, cells were reactivated on plates pre-coated with 2 μg/ml of anti-CD3 and anti-CD28 (PV1, BioXCell), for an additional 48 h, prior to adoptive transfer.
Kwong B., Rua R., Gao Y., Flickinger J J.r., Wang Y., Kruhlak M.J., Zhu J., Vivier E., McGavern D.B, & Lazarevic V. (2017). T-bet-dependent NKp46+ innate lymphoid cells regulate the onset of TH17-induced neuroinflammation. Nature immunology, 18(10), 1117-1127.
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3

Naïve 2D2 TCR-transgenic CD4+ T Cell Differentiation

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Naïve 2D2 TCR-transgenic CD4+ T cells from spleen and lymph nodes of 2D2 wild-type or 2D2 Tbx21−/− mice were electronically sorted (CD4+Vβ11+CD62Lhi), and activated under TH17 polarizing conditions with the following: anti-CD3 (2.5 μg/ml) (145-2C11, BioXCell), anti-IL-4 (20 μg/ml) (11B11, BioXCell), anti-IFN-γ (20 μg/ml) (XMG1.2, BioXCell), mIL-6 (30 ng/ml) (Miltenyi Biotec), hTGF-β1 (3 ng/ml) (Miltenyi Biotec) in the presence of irradiated wild-type splenocytes at 5:1 ratio. After 60 h of activation, mIL-23 (10 ng/ml; R&D Systems) was added. On day 5 of culture, cells were reactivated on plates pre-coated with 2 μg/ml of anti-CD3 and anti-CD28 (PV1, BioXCell), for an additional 48 h, prior to adoptive transfer.
Kwong B., Rua R., Gao Y., Flickinger J J.r., Wang Y., Kruhlak M.J., Zhu J., Vivier E., McGavern D.B, & Lazarevic V. (2017). T-bet-dependent NKp46+ innate lymphoid cells regulate the onset of TH17-induced neuroinflammation. Nature immunology, 18(10), 1117-1127.
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