Full length e cadherin

Manufactured by OriGene

Full-length E-cadherin is a recombinant protein that corresponds to the full-length sequence of the human E-cadherin protein. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in maintaining the integrity of epithelial tissues.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin antibody, by Merck Group (1 mentions) Anti zeb1 antibody, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti myc, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Anti gfp, by Merck Group (1 mentions) Anti e cadherin, by BD (1 mentions) Anti vimentin, by BD (1 mentions)

2 protocols using full length e cadherin

Immunoblot Analysis of Cellular Signaling Pathways

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The antibodies against PTRH2 (Bit1), total Erk1/2, phosphorylated, Erk1/2 (Thr-202/Tyr-204), total Akt, and activated Akt were from Cell Signaling Technology (Danvers, MA). The mouse monoclonal anti-B-actin antibodies were purchased from Sigma (St. Louis, MO) while the anti-E-cadherin antibodies were purchased from BD Biosciences (San Diego, CA). The MEK/ERK inhibitor U0126 was purchased from Calbiochem (La Jolla, CA). The full-length E-cadherin and the corresponding control constructs were obtained from Origene.

Yao X., Gray S., Pham T., Delgardo M., Nguyen A., Do S., Ireland S.K., Chen R., Abdel-Mageed A.B, & Biliran H. (2017). Downregulation of Bit1 expression promotes growth, anoikis resistance, and transformation of immortalized human bronchial epithelial cells via Erk activation-dependent suppression of E-cadherin. Biochemical and biophysical research communications, 495(1), 1240-1248.

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Immunoblotting of Epithelial-Mesenchymal Transition Markers

Cited 1 time

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The mouse monoclonal anti-myc, anti-FLAG, anti-GFP, and anti-B-actin antibodies were obtained from Sigma (St. Louis, MO), while the anti-E-cadherin and anti-vimentin antibodies were purchased from BD Biosciences (San Diego, CA). The mouse polyclonal anti-TLE1 antibody was obtained from Abcam (Cambridge, MA) while the anti-Zeb1 antibody was purchased from Santa Cruz Biotechnology, Santa Cruz, CA). The FLAG-AES and the various plasmid constructs encoding for Bit1 were generated as described previously [9 (link)]. The GFP-TLE1 and the full length E-cadherin encoding plasmids were obtained from Origene (Rockville, MD).

Yao X., Pham T., Temple B., Gray S., Cannon C., Chen R., Abdel-Mageed A.B, & Biliran H. (2016). The Anoikis Effector Bit1 Inhibits EMT through Attenuation of TLE1-Mediated Repression of E-Cadherin in Lung Cancer Cells. PLoS ONE, 11(9), e0163228.

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