Luciferase assay was performed using luciferase assay system (Promega) according to kit's manual. Briefly, HEK293T cells were transfected with luciferase reporter constructs using X-tremeGENE 9 transfection reagent (Roche). Cells were harvested in 24-48 h post-transfection. The firefly and renilla luciferase activity in cell lysates were measured using GLOMAX multi detect system (Promega).
Glomax multi detect system
Manufactured by Promega
The GloMax® Multi+ Detection System is a versatile, multi-mode microplate reader that can be used for a variety of luminescent, fluorescent, and absorbance-based assays. The system features a high-sensitivity luminometer, a monochromator-based optical system, and a temperature-controlled incubator. It is designed to provide accurate and reliable results for a wide range of life science applications.
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Lab products found in correlation
2 protocols using glomax multi detect system
1
Quantifying Luciferase Activity in HEK293T
2
Luciferase Assay for miRNA-3'UTR Interaction
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The luciferase assay was performed using the luciferase assay system (Promega) according to the manufacturer's instructions. Briefly, HEK293T cells, cultured in 12-well plates, were transfected with luciferase reporter constructs using X-tremeGENE 9 transfection reagent (Roche). Cells were harvested 24 h post-transfection. The firefly and Renilla luciferase activity in cell lysates was measured using GLOMAX multi detect system (Promega). We used 100 nM of control or hsa-miR-1 mimic (Invitrogen) and 200 ng of JARID2 3′ UTR WT or mutant vector (pmiR-GLO vector) for each well.
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