Human primary fibroblasts (ATCC® CCL-171™), Human primary Astrocytes (Science Cell Laboratories, cat #1800), T-Rex-293 cell line (RRID:CVCL_D585), L929 cell line (Sigma-Aldrich Cat# 85011425), J774 cell line (J774A.1, Sigma-Aldrich Cat# 91051511), RAW264.7 cell line (ATCC Cat# TIB-71, RRID:CVCL_0493) and THP1-Blue™ NF-κB Cells cell line (InvivoGen Cat # thp-nfkb) were cultured in DMEM (Invitrogen) supplemented with 10% heat inactivated FBS (Invitrogen), 10 mM HEPES buffer pH 7.2, 2 mM L-glutamine, 50 μg/ml of penicillin and streptomycin (Invitrogen). Human Astrocytes (ScienCell Research Laboratories) were cultured in Astrocyte Medium (ScienCell Research Laboratories). Cells were incubated at 37°C in 5% CO2. The Toxoplasma strains used in this study are listed in Table S1 . All T. gondii strains were maintained in vitro by serial passage on monolayers of HFFs. T. gondii strains were maintained in vitro by serial passage on monolayers of HFFs. The strains used in this study were Pru∆ku80, RH∆ku80 and 76K-GFP-LUC (gift of M. Grigg, National Institutes of Health, Bethesda, MD). The cultures were free of mycoplasma, as determined by qualitative PCR.
Thp1 blue nf κb cells cell line
Manufactured by InvivoGen
THP1-Blue™ NF-κB Cells is a human monocytic cell line that stably expresses a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an NF-κB-inducible promoter. This cell line can be used to monitor NF-κB activation.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions)
Raw264.7 cell line, by American Type Culture Collection (2 mentions)
L929 cell line, by Merck Group (2 mentions)
Atcc ccl 171, by American Type Culture Collection (2 mentions)
T rex 293 cell line, by Merck Group (2 mentions)
J774 cell line j774a 1, by Merck Group (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Astrocyte medium, by ScienCell (2 mentions)
Human astrocyte, by ScienCell (2 mentions)
2 protocols using thp1 blue nf κb cells cell line
1
Culturing Primary and Cell Line Cells for Toxoplasma Study
2
Culturing Primary and Cell Line Cells for Toxoplasma Study
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Human primary fibroblasts (ATCC® CCL-171™), Human primary Astrocytes (Science Cell Laboratories, cat #1800), T-Rex-293 cell line (RRID:CVCL_D585), L929 cell line (Sigma-Aldrich Cat# 85011425), J774 cell line (J774A.1, Sigma-Aldrich Cat# 91051511), RAW264.7 cell line (ATCC Cat# TIB-71, RRID:CVCL_0493) and THP1-Blue™ NF-κB Cells cell line (InvivoGen Cat # thp-nfkb) were cultured in DMEM (Invitrogen) supplemented with 10% heat inactivated FBS (Invitrogen), 10 mM HEPES buffer pH 7.2, 2 mM L-glutamine, 50 μg/ml of penicillin and streptomycin (Invitrogen). Human Astrocytes (ScienCell Research Laboratories) were cultured in Astrocyte Medium (ScienCell Research Laboratories). Cells were incubated at 37°C in 5% CO2. The Toxoplasma strains used in this study are listed in Table S1 . All T. gondii strains were maintained in vitro by serial passage on monolayers of HFFs. T. gondii strains were maintained in vitro by serial passage on monolayers of HFFs. The strains used in this study were Pru∆ku80, RH∆ku80 and 76K-GFP-LUC (gift of M. Grigg, National Institutes of Health, Bethesda, MD). The cultures were free of mycoplasma, as determined by qualitative PCR.
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