Chlorpyriphos

Manufactured by Merck Group

Sourced in United States

Chlorpyriphos is a chemical compound used as an active ingredient in various agricultural and household insecticide products. It functions as an organophosphate insecticide, primarily effective against a broad range of insects and pests. The core function of chlorpyriphos is to act as a pesticide, disrupting the nervous system of target organisms.

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Lab products found in correlation

3 protocols using chlorpyriphos

Insecticide Preparation and Dosing

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DDT (1,1,1-trichloro-2,2-bischlorophenylethane, Lot: #BCBW0671), chlorpyriphos (Lot: #SZBD343XV), were purchased from Sigma-Aldrich. Chlorantraniliprole (Lot: #CEAG210011) was purchased from 3A Chemicals. Insecticides were dissolved in acetone. Solutions with 2, 10, 50, 100, 200, 500, 1000, 2000 μg of DDT per ml yielding 0.02, 0.1, 0.5, 1, 2, 5, 10 and 20 μg per vial (according to Abdu-Allah et al. [32 (link)]), three chlorpyrifos solutions with the concentrations of 1 μg/ml yielding 0.01 μg per vial (according to Wang et al. (31)) and chlorantraniliprole solutions with 5000 μg chlorantraniliprole per ml yielding 50 μg per vial (according to Ma et al. [33 (link)]) were prepared.

Yang J., Wang Y., El Wakil A, & Moussian B. (2024). Extra-corporeal detoxification in insects. Heliyon, 10(7), e28392.

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Analytical Standards for Environmental and Toxicological Analysis

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LC grade solvents, methanol (MeOH), acetonitrile (ACN), dichloromethane (CH₂Cl₂), hexane, and octanol were purchased from VWR BDH Chemicals HiPerSolv Chromanorm (Milan, Italy). Milli-Q water was produced using a Millipore Direct-Q 3 UV purification system (Millipore Corp., Milan, Italy). Fused silica capillaries of various internal (I.D.) and external (O.D.) diameters were purchased from Polymicro Technologies (Phoenix, USA).
Pesticides: chlorpyriphos (CAS 2921-88-2; PESTANAL, analytical standard 99.9% purity), imazalil (CAS 35554-44-0; PESTANAL, analytical standard 99.8% purity), and benomyl (CAS 17804-35-2; PESTANAL, analytical standard, ≥ 98.0% purity) were supplied by Sigma Aldrich. imazalil and benomyl stock solutions were prepared gravimetrically at 1000 mg/L concentration in ACN. Chlorpyrifos stock solution was prepared gravimetrically at a concentration of 1000 mg/L in ACN. Solutions of chlorpyrifos at 50, 100, and 500 mg/L were prepared by diluting the stock solution with the same solvent.
Cocaine (CAS 50-36-2; Cerilliant, Certified Reference Material) stock solution at a concentration of 1000 mg/L in ACN was provided and manipulated by the Toxicology Laboratory A.S.T. AV1, (Pesaro, Italy).
Caffeine (CAS 58-08-2; analytical standard > 99.0% purity) solution was prepared gravimetrically at 1000 mg/L concentration in ACN.

Arigò A., Famiglini G., Marittimo N., Agostini M., Renzoni C., Palma P, & Cappiello A. (2023). Extractive-liquid sampling electron ionization-mass spectrometry (E-LEI-MS): a new powerful combination for direct analysis. Scientific Reports, 13, 6429.

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Neurotoxic Pollutants Screening in Zebrafish

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Thirty neurotoxic pollutants, from 5 chemical groups (herbicide, insecticide, metal elements, non-metal elements, and toxins) were selected (Table 1). The following compounds were purchased from Sigma-Aldrich (St. Louis, MO, USA): acephate, acetamiprid, aldicarb, cadmium(II) sulfate (Cd), carbaryl, carbofuran, chlorpyriphos, clothianidin, diazinon, dichlorvos, endosulfan, esfenvalerate, fenitrothion, malathion, manganese(II) chloride (Mn), mercury(II) chloride (Hg), lead(II) nitrate (Pb), permethrin, selenium dioxide (Se), sodium fluoride (fluorine), thiamethoxam and β-cyfluthrin. Atrazine and deltamethrin were purchased from Riedel-de Haën (Germany). Dinotefuran and fenvalerate were purchased from Supelco™ Analytical (USA). Glyphosate was purchased from Santa Cruz Biotechnology (USA), whereas domoic acid, okadaic acid and saxitoxin were purchased from Cifga lab SA (Spain). CAS numbers of each chemical are provided in Table 1. Stock solutions of each compound were prepared on the day of the experiment. Solutions were either prepared directly in fish water or in dimethyl sulfoxide (DMSO), according to compound solubility.
Experimental solutions were prepared in fish water from the stock solutions.
DMSO was added to all conditions, including control, to a final concentration of 0.1%, which has been previously found not to affect larvae behavior (Faria et al., 2019c) .

Faria M., Wu X., Luja-Mondragón M., Prats E., Gómez-Oliván L.M., Piña B, & Raldúa D. (2020). Screening anti-predator behaviour in fish larvae exposed to environmental pollutants. The Science of the total environment, 714.

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