Anti-RPE65 (ab231782), anti-RIP3 (phosphor s232, ab195117), and anti-Rodopsin (ab5417) were purchased from abcam (Boston, MA, USA). Other antibodies used in this study were obtained from the following sources: anti-cleaved caspase 3 (#9664), anti-RIP1 (#3493), and anti-mouse β-actin (#3700) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Mouse anti-GFAP (glial fibrillary acidic protein, specific for glia/Müller cells) was obtained from BD Biosciences (San Jose, CA, USA). Anti-mouse Iba1 was purchased from FUJIFILM WAKO Chemicals, U.S.A. Corporation (019-19741, Richmond, VA, USA). Goat anti-rabbit IgG HRP (sc2004) and rabbit anti-goat IgG HRP (sc2768) were sourced from Santa Cruz (Santa Cruz Biotechnology, CA, USA). Anti-Mouse IgG HRP Conjugate (W402B) was obtained from Promega (Madison WI, USA). Anti-mouse Alexa 488 and anti-rabbit Alexa 594 were obtained from Vector Laboratories, Inc. (Burlingame, CA, USA).
Anti rip1 3493
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-RIP1 (#3493) is a primary antibody that recognizes the receptor-interacting serine/threonine-protein kinase 1 (RIP1), a key mediator of cell death and inflammation pathways. This antibody can be used for applications such as western blotting and immunoprecipitation to detect and study the expression and function of RIP1 in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions)
Anti cleaved caspase 3 9664, by Cell Signaling Technology (1 mentions)
Mouse anti gfap, by BD (1 mentions)
Anti mouse alexa 488, by Vector Laboratories (1 mentions)
Anti rabbit alexa 594, by Vector Laboratories (1 mentions)
Rabbit anti goat igg hrp sc 2768, by Santa Cruz Biotechnology (1 mentions)
Mouse anti β actin, by Cell Signaling Technology (1 mentions)
Trypsin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
2 protocols using anti rip1 3493
1
Antibody Acquisition for Research
2
Quantification of Apoptosis Markers in HT22 Cells
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Antibodies: anti-βactin (20536-1-AP), anti-Tubulin (10094-1-AP), anti-GAPDH (60004-1-Ig), Goat antimouse antibody (SA0001-1), Goat anti-rabbit antibody (SA0001-1) were purchased from Proteintech company in the United States. Anti-Menin (ab31902) was purchased from Epitomics-an Abcam Company in the United States. Anti-p-RIP1 (65746) and anti-RIP1 (3493) were obtained from Cell Signaling Technology in the United States. Anti-P-RIP3 (AF7443), anti-RIP3 (AF7942), anti-P-MLKL (AF7420), and anti-MLKL (AF7412) were purchased from A nity Biosciences in the United States.
Regents: Corticosterone (C0388, TCL Chemical Industry Development Co., Ltd., Shanghai). 4-Octyl Itaconate (3133-16-2, MedChemExpress, USA), DMSO (D2650, Sigma, USA), Trypsin (T4049, Sigma, USA), CCK-8 (Dongren Chemical Technology Co., Ltd., Shanghai), MSD (Dongren Chemical Technology Co., Ltd., USA) 2.2 Cells culture HT22 cells were obtained from Shunran Biotechnology Co., Ltd (Shanghai, China) and were incubated in a DMEM medium with 10% FBS and 1% Penicillin streptomycin. And cultured in the 37 centigrade, 5% CO2 incubator. Replace DMEM medium every 1-2 days. Subculture and frozen cells when the cells are in the logarithmic growth phase.
Regents: Corticosterone (C0388, TCL Chemical Industry Development Co., Ltd., Shanghai). 4-Octyl Itaconate (3133-16-2, MedChemExpress, USA), DMSO (D2650, Sigma, USA), Trypsin (T4049, Sigma, USA), CCK-8 (Dongren Chemical Technology Co., Ltd., Shanghai), MSD (Dongren Chemical Technology Co., Ltd., USA) 2.2 Cells culture HT22 cells were obtained from Shunran Biotechnology Co., Ltd (Shanghai, China) and were incubated in a DMEM medium with 10% FBS and 1% Penicillin streptomycin. And cultured in the 37 centigrade, 5% CO2 incubator. Replace DMEM medium every 1-2 days. Subculture and frozen cells when the cells are in the logarithmic growth phase.
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