Dulbecco s modified eagle s medium dmem high glucose medium

Cisplatin was purchased from Shandong Boyuan Pharmaceuti-cal Co., Ltd. (99.8%, Jinan, Shandong, China). Poly(ethylene glycol) monomethyl ether (mPEG_5k-OH, Mn = 5000 g/mol), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, 98.0%), and Hoechst 33,258 (≥98.0%) were bought from Sigma-Aldrich. Cur, L-ascorbic acid sodium salt (SA, 99.0%), hexamethylene diisocyanate (HDI, 99.0%), dibutyltin dilaurate (95.0%), L-Lysine diisocyanate (LDI, 95.0%), and hydrogen peroxide solution (H₂O_2, 30. wt% in water) were obtained from Aladdin Chemistry Co. Ltd. (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium, phosphate-buffered saline (PBS) and trypsin were purchased from GE Healthcare Life Sciences. Gibco Fetal Bovine Serum (FBS) was bought from Thermo Fisher Scientific Inc. (Shanghai, China). All chemical reagents were of analytical grade, without further purification, if not mentioned otherwise. Dichloromethane (DCM, ≥99.5%, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), dimethyl sulfoxide (DMSO, ≥99.0%, Sinopharm Chemical Reagent Co.,Ltd., Shanghai, China), and N,N-Dimethylformamide (DMF, ≥99.5%, Sinopharm Chemical Reagent Co.,Ltd., Shanghai, China) were dried with calcium hydride (CaH₂, 95.0%, Aladdin Chemistry Co. Ltd., Shanghai, China) for 2 weeks before further purification.

Dextran10 k, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, 98%), sodium metaperiodate (NaIO₄, ≥99%), doxorubicin hydrochloride (DOX·HCl, 98%), and Hoechst 33,258 (≥98%) was obtained from Sigma-Aldrich (Shanghai, China). Cisplatin (99.8%) was bought from Shandong Boyuan Pharmaceuti-cal Co., Ltd. (Jinan, Shandong, China). Hydrogen peroxide solution (H₂O₂, 30 wt% in water), succinic anhydride (99%), triethylamine (TEA, ≥99.5%), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl, ≥98%) were purchased from Aladdin Chemistry Co. Ltd. (Shanghai, China). Phosphate-buffered saline (PBS), dulbecco’s modified eagle’s medium (DMEM)/high-glucose medium, and trypsin were obtained from GE Healthcare Life Sciences (Beijing, China). Gibco Fetal Bovine Serum (FBS) was purchased from Thermo Fisher Scientific Inc. (Shanghai, China). Lyso-tracker was purchased from Shanghai Biyuntian Biological Co., Ltd. (Shanghai, China). All chemical reagents were used as received without purification if not mentioned otherwise. Dimethyl sulfoxide (DMSO, ≥99%) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), and distilled followed by dried with calcium hydride (CaH₂) for 2 weeks.