The following chemicals were employed in our research: FGF2 was supplied from the biotechnology pharmaceutical engineering lab at Wenzhou Medical University and synthesized based on previous work. Masson Staining Kit and BCA Kit are both available from Thermo Fisher Scientific in the United States and Solarbio Science in Beijing, China, respectively. The following companies provided the specific primary antibodies that were needed: HO-1 (10701-1-AP), NQO1 (11451-1-AP), SOD1 (24127-1-AP), AMPK (66536-1-lg), NFE2L2 (16396-1-AP), GAPDH (60004-1-lg) and Histone-H3 (17168-1-AP) from Proteintech Group; SLC7A11 (DF12509) and KLF2 (DF13602) from Affinity; GPX4 (381958) from ZEN Bio; p-AMPK (2535) from abcam; HDAC5 (A0632) and p-HDAC5 (AP0202) from Abclonal; 4-HNE (MAB3249-SP) from R&D Systems. CD31 (sc-376764) antibody from Santa Cruz. The secondary IgG antibody coupled with horseradish peroxidase (HRP) was purchased from Santa (Cruz, CA, USA).
Ho 1 10701 1 ap
Manufactured by Proteintech
Sourced in China
HO-1 (10701-1-AP) is a primary antibody product offered by Proteintech. It is intended for use in common research applications such as Western blotting, immunohistochemistry, and immunofluorescence. The product targets the heme oxygenase 1 (HO-1) protein.
Automatically generated - may contain errors
Lab products found in correlation
Bca kit, by Thermo Fisher Scientific (1 mentions)
Nfe2l2 16396 1 ap, by Proteintech (1 mentions)
Histone h3 17168 1 ap, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Np 40, by Beyotime (1 mentions)
Valeric acid, by Merck Group (1 mentions)
Isobutyric, by Merck Group (1 mentions)
Butyric, by Merck Group (1 mentions)
Nf κb p65 10745 1 ap, by Proteintech (1 mentions)
Keap1 10503 2 ap, by Proteintech (1 mentions)
Nrf2 16396 1 ap, by Proteintech (1 mentions)
β actin gb11001, by Wuhan Servicebio Technology (1 mentions)
Goat anti rabbit igg h l alexa fluor 488 ab150077, by Abcam (1 mentions)
Ab6671, by Abcam (1 mentions)
Bs 2513r, by Bioss Antibodies (1 mentions)
Lps l2630, by Merck Group (1 mentions)
β actin bs 0061r, by Bioss Antibodies (1 mentions)
Bs 0812r, by Bioss Antibodies (1 mentions)
Ab15323, by Abcam (1 mentions)
Ab13556, by Abcam (1 mentions)
5 protocols using ho 1 10701 1 ap
1
Comprehensive Protein Expression Analysis
2
Protein Complex Enrichment and Analysis
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PDLSCs were lysed with NP-40 (Beyotime, Nantong, China) on ice for 10 min, and the supernatant was collected after centrifugation. The supernatant samples were incubated with the primary antibodies (2.5 μg) and Protein A/G Plus Agarose (sc-2003, Santa) at 4°C overnight. And the primary antibodies used in this study for enriching the protein complex were presented below: Nrf2 (16396-1-AP, Proteintech), Akt (10176-2-AP, Proteintech), HO1 (10701-1-AP, Proteintech), SOD2 (24127-1-AP, Proteintech). After immunoprecipitation, the immunocomplexes were washed three times with NP-40 lysis buffer, collected after centrifugation, and then re-suspended with SDS-loading buffer, and boiled for 5 min for western blotting. The primary antibodies used for western blotting were presented below: Nrf2 (66504-1-lg, Proteintech), Akt (60203-2-lg, Proteintech), HO1 (66743-1-lg, Proteintech), SOD2 (66474-1-lg, Proteintech).
3
Ferulic Acid Modulates Nrf2/Keap1/HO-1 and NF-κB Signaling
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Ferulic acid (FA, purity ≥ 98 %) was obtained from Dalian Meilun Biotechnology (Dalian, China). Acetic, propionic, butyric, isobutyric, isovaleric, and valeric acid standards were provided by the Sigma-Aldrich Chemical Co. Ltd (St. Louis, MO, USA).
The antibodies of Nuclear factor E2-related factor 2 (Nrf2,16396-1-AP), Kelch-like ECH-associated protein 1(Keap1,10503-2-AP), Heme oxygenase-1(HO-1,10701-1-AP), and Nuclear factor kappa B p65 (NF-κB p65,10745-1-AP) were purchased from Proteintech (Wuhan, China). p38 MAPK (A0227), Phospho-p38 MAPK (P-p38, AP0057), Phospho-Nuclear factor kappa B p65 (P-p65, AP0446) were purchased from ABclonal (Wuhan, China). The β-actin (GB11001) was the production of Servicebio (Wuhan, China).
The antibodies of Nuclear factor E2-related factor 2 (Nrf2,16396-1-AP), Kelch-like ECH-associated protein 1(Keap1,10503-2-AP), Heme oxygenase-1(HO-1,10701-1-AP), and Nuclear factor kappa B p65 (NF-κB p65,10745-1-AP) were purchased from Proteintech (Wuhan, China). p38 MAPK (A0227), Phospho-p38 MAPK (P-p38, AP0057), Phospho-Nuclear factor kappa B p65 (P-p65, AP0446) were purchased from ABclonal (Wuhan, China). The β-actin (GB11001) was the production of Servicebio (Wuhan, China).
4
Amentoflavone Modulates Neuroinflammation
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Amentoflavone (THD256) was purchased from Ronghe Pharmaceutical Co., Ltd. (Shanghai, China). LPS (L2630) was purchased from Sigma-Aldrich (St Louis, MO, USA). Primary antibodies of TLR4 (ab13556), MyD88 (ab135693), iba-1 (ab178847), TNF-α (ab6671), iNOS (ab15323), and goat antirabbit IgG H&L (Alexa Fluor® 488) (ab150077) were purchased from Abcam (San Francisco, CA, USA). Primary antibodies of IL-1β (bs-0812R), p-IκB (bs-2513R), NF-κB p65 (bs-0465R), Keap1 (bs-3648R), Nrf2 (bs-1074R), PCNA (bs-0754R), and β-actin (bs-0061R) were purchased from Bioss Biotechnology Co., Ltd. (Woburn, MA). HO-1 (10701-1-AP) was purchased from ProteinTech Group (Wuhan, China). Peroxidase-conjugated goat antirabbit IgG (H+L) (ZB-2301) was purchased from Zhongshan Jinqiao Biotechnology Co., Ltd. (Beijing, China). Other general agents were commercially available.
5
Protein Extraction and Western Blot Analysis
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In brief, proteins were collected using lysis buffer (Cat No. P0013B, Beyotime, Shanghai, China), and the protein concentration was measured using a BCA Protein Assay Kit (PC0020, Solarbio Science & Technology, Beijing, China). Before incubation with the antibody, equal quantities of protein samples were separated by denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes. A ChemiDoc MP Imaging System was used to image the blots (Bio-Rad, Hercules, CA, USA). p-SAPK/JNK (Thr183/Tyr185; #4685), SAPK/JNK (#9252), p-Src (Ser17; #12432), p-Stat3 (Tyr705; #9145), mTOR (2065AP), p-mTOR (Ser2448; #5536), p-Akt (Ser473; #4060), p-GSK3β (Ser9; #5558), PARP (#9532) and cleaved-PARP (#94885) were provided by Cell Signaling Technology. Src (11097-1-AP), AKT (11097-1-AP), Stat3 (10253-2-AP), ERK1/2 (16443-1-AP), GSK3β (#12456), GAPDH (10494-1-AP), P53 (10442-1-AP), Bcl2 (10068-1-AP), Bax (50599-2-19), IL-6 (21865-1-AP), Nrf2 (16396-1-AP) and HO-1 (10701-1-AP) were purchased from Proteintech. Immobilon® PVDF membranes were provided by Thermo Fisher Scientific (Waltham, MA, USA) and Merck KGaA (Darmstadt, Germany), respectively.
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