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Electron multiplier ccd camera

Manufactured by Hamamatsu Photonics
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Sourced in Sweden, Japan

The Electron Multiplier CCD (Charge-Coupled Device) Camera is a type of camera that utilizes an electron multiplier to amplify the signal from the CCD sensor. The core function of this camera is to provide high sensitivity and low noise imaging, making it suitable for applications that require the detection of low-light signals.

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Lab products found in correlation

Ix81 fluorescence microscope, by Olympus (2 mentions) Uapon 100xotirf, by Olympus (1 mentions) Imagem, by Hamamatsu Photonics (1 mentions) Uplansapo, by Olympus (1 mentions)

Close spelling variants of this product

CCD camera (119 citations) ImagEM Electron multiplier (EM) CCD camera (3 citations)

3 protocols using electron multiplier ccd camera

1

Single-Molecule Fluorescence Microscopy Protocol

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A combination of an inverted fluorescence microscope
IX71 (Olympus
Co., Tokyo, Japan), an objective lens UApo N 100XOTIRF (Olympus Co.),
a Samba 532-nm laser unit (Cobolt Inc., Solna, Sweden), an Electron
Multiplier-CCD camera ImagEM (HAMAMATSU Photonics K.K., Shizuoka,
Japan) was used as a TIRFM system. A biotinylated probe-DNA-immobilized
cover glass of the reaction cell was placed in contact with the immersion
oil on the objective lens of TIRFM. The cover glass was irradiated
by a laser light at 532 nm from the lower side through the objective
lens, resulting in the generation of an evanescent field on the upper
surface of the glass plate (Figure 2A). When the Cy3-labeled target DNAs were added to
the buffer solution in the reaction cell, CCD-camera images were recorded
by laser irradiation with 60.53 ms exposure time. Cy3-labeled DNAs
hybridized with probe DNAs immobilized on the glass plate appeared
as bright spots in the images (Figure 2B). Time-lapse images were recorded at 5, 10, 20, 30,
40, 60, and 70 s. Software MetaMorph was used for analyzing the resultant
images.
Yazawa K, & Furusawa H. (2018). Probing Multiple Binding Modes of DNA Hybridization: A Comparison between Single-Molecule Observations and Ensemble Measurements. ACS Omega, 3(2), 2084-2092.
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2

DNA Nanostructure Fluorescence Imaging

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The DNA nanostructure containing biotin modified staple strands was loaded to a µ-Slide I0.2 Luer ibidi-Treat pretreated by biotinylated bovine serum albumin and neutravidin, adsorbed for 15 min at room temperature, and then washed with a buffer (pH 7.5) containing 25 mM HEPES, 10 mM MgCl2, and 0.4 M KCl. The fluorescence of the samples was observed using an IX-81 fluorescence microscope (Olympus, Tokyo, Japan) equipped with a 20× objective lens and Xe lamp. Fluorescence images were acquired with electron multiplier CCD camera (Hamamatsu Photonics K.K., Shizuoka, Japan) at ambient temperature. After the fluorescence of fluorescein, rhodamine, and Alexa Fluor 647 were sequentially monitored, the sample was washed with a buffer (pH 6.0) containing 25 mM HEPES, 10 mM MgCl2, and 0.4 M KCl. The fluorescence images of them were taken at the same position. After that, the sample was washed again with a buffer (pH 7.5) containing 25 mM HEPES, 10 mM MgCl2, and 0.4 M KCl, and the fluorescence images were taken.
Konishi H., Nakata E., Komatsubara F, & Morii T. (2023). Controlled Assembly of Fluorophores inside a Nanoliposome. Molecules, 28(2), 911.
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3

Fluorescent Monitoring of H2O2 and NO

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HEK293 cells were seeded onto a poly l-lysine-coated 35 mm glass-bottom dish (IWAKI) and incubated for 1 h. Cells were washed with HBS containing 107 mM NaCl, 6 mM KCl, 11.5 mM glucose, 20 mM HEPES, 1.2 mM MgSO4, and 2 mM CaCl2, pH 7.4, and 200 μL HBS were added to the dish. Fluorescence was observed using an IX-81 fluorescence microscope (Olympus) equipped with a 20× objective lens (UPlanSApo) and Xe lamp. Fluorescence images when excited at 403 nm and 480 nm was acquired with electron multiplier CCD camera (Hamamatsu Photonics K.K.) by using a 381 nm to 415 nm and a 457 nm to 507 nm band-pass filter for excitation, respectively, and a 505 nm dichroic mirror, and 522 nm to 548 nm band-pass filter for emission. For time-lapse imaging to observe the fluorescence response to H2O2, 100 μL of 1.5 mM of H2O2 dissolved in HBS were gently added to the dish. In the case of NO, 500 μM NOC7 dissolved in HBS was applied. Time-lapse images were acquired every 5 min.
Tajima S., Nakata E., Sakaguchi R., Saimura M., Mori Y, & Morii T. (2022). A two-step screening to optimize the signal response of an auto-fluorescent protein-based biosensor. RSC Advances, 12(24), 15407-15419.
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