The largest database of trusted experimental protocols

Pcmv6 c myc

Manufactured by OriGene

PCMV6-c-myc is a mammalian expression vector that contains the human c-myc gene under the control of the CMV promoter. This vector can be used for the expression of the c-myc protein in various cell lines.

Automatically generated - may contain errors

2 protocols using pcmv6 c myc

1

Construction of TNBC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
pCMV6-E2F1, pCMV6-c-myc, and pCMV6-XL5 empty plasmid was purchased from Origene (Rockville, MD). MiR-301a hairpin antagomirs were obtained from GeneCopoeia Inc. (Guangzhou, China). All transfections were conducted using Lipofectamine 2000 according to the manufacturer's instructions (Invitrogen). miR-301a-expressing retroviruses, Cip2a-expressing retroviruses were purchased from GENECHEM (Shanghai, China). The Cip2a short hairpin RNA (shRNA) lentivirus vectors were generated by GeneCopoeia Inc. (Guangzhou, China). The target sequences of Cip2a shRNA was 5'-CUGUGGUUGUGUUUGCACUTT-3'. For selection of TNBC stable cell lines, retroviruses were transduced into BT549 and MDA-MB-231 cells in the presence of polybrene (6 μg/mL, Sigma). Cells were selected with 1 μg/mL puromycin for 7 days.
+ Open protocol
+ Expand
2

Lentiviral Silencing and Overexpression of NNMT, SIRT1, c-myc

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral vectors expressing nontargeting pLKO.1 control shRNA (SCH002), and two shRNA constructs targeting NNMT-shRNA1 (TRCN0000035224) and -shRNA2 (TRCN0000035225) were obtained from Sigma-Aldrich. Expression plasmid for pCMV6-NNMT, pCMV6-SIRT1, pCMV6-c-myc, and pCMV6-XL5 empty plasmid were purchased from Origene (Rockville, MD). For cell transfection, Lipofectamine 2000 reagent (Invitrogen) was used according to the manufacturer’s instructions. After 72 h infection, stably expressing or knockdown cells were selected in RPMI 1640 medium containing 1 μg/mL puromycin.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!