2 5 di tert butylhydroquinone tbhq

The Opti-MEM reduced serum media, fetal bovine serum, penicillin–streptomycin, Fura-2 AM, Fluo-4 AM, Mag-Fluo-4 AM, and the Annexin V-FITC staining Kit were from ThermoFisher Scientific (Burlington, ON, Canada). l-asparaginase (ASNase, ab73439) and 2,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM) were from Abcam (Toronto, ON, Canada). 2,5-Di-tert-butylhydroquinone (TBHQ) and tetracaine (Tet) were from Sigma (Oakville, ON, Canada). Xestospongin-C (XeC) was from Bio-Techne (Oakville, ON, Canada). Antibodies to OPRM1 (D-12), Bid (B-3), caspase-3 (E-8), caspase-12 (1611), poly(ADP-ribose) polymerase-1 (PARP1, F-2), GAPDH (0411), calpain-1 (6C-12), cytochrome C (H-104), voltage-dependent anion-selective channel 1 (VDAC-1, B-6), tubulin (D-10) and actin (I-19) were from Santa Cruz Biotech. (Dallas, TX, USA). The antibody against cleaved caspase-3 (D1–75) was from Cell Signaling (Whitby, ON). Trypan blue was from Life Technologies (Burlington, ON, Canada). Alamar blue was from Life Technologies (ON, Canada). Calpeptin was from Calbiochem, CA, USA.

RPMI 1640 media, fetal bovine serum, penicillin-streptomycin, Mag-Fluo-4 AM, Rhod-2 AM, Fluo-4 AM, Annexin V-FITC staining kit, Image-IT live mitochondria permeability transition pore assay kit, MitoSOX Red, MitoTracker green and DCFDA were from Thermo Fisher Scientific (Burlington, ON, Canada). L-asparaginase (ab73439) was from Abcam (Toronto, ON, Canada). 2,5-di-tert-butylhydroquinone (TBHQ) was from Sigma (Oakville, ON, Canada). Xestospongin-C (XeC), ruthenium red (RuR), and cyclosporine A (CsA) were from Bio-Techne (Oakville, ON, Canada). HAP1 (D-12) and actin (I19) antibodies, and Mito-Tempo were from Santa Cruz Biotech. (Dallas, TX, United States of America).