Preparative liquid chromatography (prep-LC) was performed using an LC-Forte/R system (YMC, Kyoto, Japan) equipped with a YMC-DispoPack cartridge (ODS, 30 g) (YMC, Kyoto, Japan). For column chromatography, Silica gel (230–400 mesh) (Merck, Darmstadt, Germany), Kromasil 100-5-C18 (Nouryon, Bohus, Sweden), and Sephadex LH-20 (Merck, Darmstadt, Germany) were used. Chromatography was performed on a Kiesel gel 60 F254 thin-layer chromatography (TLC) plate (Merck, Darmastdt, Germany) and RP-18 F254s TLC plates (Merck, Darmastdt, Germany); detection was performed via being sprayed with 10% H2SO4 solution and heated with a UV (254 nm and 356 nm). 1H, 13C NMR, and 2D NMR experiments were performed on a Bruker AVANCE II 400 (400 MHz for 1H NMR and 100 MHz for 13C NMR) NMR spectrometer (Bruker, Karlsruhe, Germany) [43 (link)]. MS data was obtained by the Agilent 6530 accurate-mass Q-TOF LC–MS instrument (Agilent Technologies, Santa Clara, CA, USA) [44 (link)]. The information of isolated compounds from S. neoserrata Nakai was obtained from NCBI PubChem database (https://pubchem.ncbi.nlm.nih.gov/ accessed on 30 June 2023). All chemical reagents were purchased from commercial suppliers, Sigma Chemical Company (St. Louis, MO, USA).
Lc forte r system
Manufactured by YMC
Sourced in Japan
The LC-Forte/R system is a liquid chromatography instrument designed for analytical and preparative applications. It provides essential chromatographic functionality for separating and analyzing complex mixtures. The system includes a pump, an autosampler, a column compartment, and a variety of detectors to support diverse analytical needs.
Automatically generated - may contain errors
Lab products found in correlation
Kieselgel 60 f254, by Merck Group (2 mentions)
Avance 2 400, by Bruker (1 mentions)
Rp 18 f254s tlc plates, by Merck Group (1 mentions)
230 400 mesh silica gel, by Merck Group (1 mentions)
Sephadex lh 20, by Merck Group (1 mentions)
Avance neo 600, by Bruker (1 mentions)
Sephadex lh 20 gel, by GE Healthcare (1 mentions)
Kieselgel 60, by Merck Group (1 mentions)
Triart c18 column, by YMC (1 mentions)
2 protocols using lc forte r system
1
Preparative Liquid Chromatography of Phytochemicals
2
Spectroscopic Characterization of Natural Compounds
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NMR spectra, including
2D NMR (1H-1H COSY, HSQC, and HMBC), were obtained
using a Bruker Avance Neo 600 (Bruker, Germany) spectrometer at 600
MHz at the Central Laboratory of Kangwon National University (Chuncheon,
Korea). Column chromatography procedures were performed on silica
gel Kieselgel 60 (40–60 μm, 230–400 mesh, Merck,
Germany) and Sephadex LH-20 gel (18–111 μm, GE Healthcare,
Sweden). Thin-layer chromatography (TLC) was performed on precoated
silica gel plates (Kieselgel 60 F254 Merck, Germany) and
RP-C18 plates (Kieselgel 60 F254s, Merck, Germany).
Spots were detected by TLC using UV light or H2SO4-EtOH (v/v) spray followed by heating. Multiple preparative HPLC
was performed on a LC-Forte/R system equipped with a YMC UV/Vis detector
(YMC, Shimogyo-ku, Japan) using a YMC-Actus Triart C18 column
(s-5 μm, 12 nm, 20 × 250 mm2) at a flow rate
of 50 mL/min and monitored at 210 nm. Extra high purity solvents were
purchased from DaeJung (Sicheung, Korea) for extraction, fractionation,
and separation.
2D NMR (1H-1H COSY, HSQC, and HMBC), were obtained
using a Bruker Avance Neo 600 (Bruker, Germany) spectrometer at 600
MHz at the Central Laboratory of Kangwon National University (Chuncheon,
Korea). Column chromatography procedures were performed on silica
gel Kieselgel 60 (40–60 μm, 230–400 mesh, Merck,
Germany) and Sephadex LH-20 gel (18–111 μm, GE Healthcare,
Sweden). Thin-layer chromatography (TLC) was performed on precoated
silica gel plates (Kieselgel 60 F254 Merck, Germany) and
RP-C18 plates (Kieselgel 60 F254s, Merck, Germany).
Spots were detected by TLC using UV light or H2SO4-EtOH (v/v) spray followed by heating. Multiple preparative HPLC
was performed on a LC-Forte/R system equipped with a YMC UV/Vis detector
(YMC, Shimogyo-ku, Japan) using a YMC-Actus Triart C18 column
(s-5 μm, 12 nm, 20 × 250 mm2) at a flow rate
of 50 mL/min and monitored at 210 nm. Extra high purity solvents were
purchased from DaeJung (Sicheung, Korea) for extraction, fractionation,
and separation.
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