Dulbecco’s Modified Eagle Medium (DMEM) culture medium with high glucose was purchased from Hyclone (American), and FBS was purchased from Gibco (American). gallic acid monohydrate, FeCl3, MnCl2·4H2O and CaCl2 were purchased from Aladdin (China). 5-FAM cadaverine was purchased from Fanbo Biochemicals Co. Ltd. The following antibodies were purchased commercially from Abcam (Britain): anti-HSP70 Ab, anti-HSP90 Ab, anti-Bcl-2 Ab, anti-Bax Ab, anti-cleaved N-terminal GSDME Ab, anti-Cleaved Caspase-3 Ab and anti-GAPDH Ab. DCFH-DA, Cell Counting Kit-8 (CCK-8), BCA Protein Quantitation Kit, Caspase 3 Activity Assay Kit, ATP Assay Kit and PI/calcein-AM were acquired from Beyotime Biological Co., Ltd. (Shanghai, China). The female Balb/c nude mice (4–6 weeks) were obtained from Charles River (Shanghai China).
Anti bax ab
Manufactured by Abcam
Sourced in United States
Anti-Bax Ab is a primary antibody that specifically recognizes the Bax protein. Bax is a pro-apoptotic member of the Bcl-2 protein family and plays a key role in the regulation of apoptosis. The Anti-Bax Ab is a useful tool for the detection and study of Bax protein expression and function in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti bcl 2 ab, by Abcam (2 mentions)
Anti gapdh ab, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Female balb c nude mice, by Charles River Laboratories (1 mentions)
Cell cycle assay kit, by Beyotime (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
5aza 2dc, by Merck Group (1 mentions)
Anticleaved caspase 3 ab, by Cell Signaling Technology (1 mentions)
Anti ki67 ab, by Abcam (1 mentions)
Annexin 5 fitc cell apoptosis assay kit, by Beyotime (1 mentions)
Cck 8, by Dojindo Laboratories (1 mentions)
2 protocols using anti bax ab
1
DMEM-based Cell Culture Protocol
2
Antibody Panel for Cellular Signaling Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were used in this study: anti-CNTF Ab (Abcam Cambridge, MA, USA, Cat. # ab46172), anti-BAX Ab (Abcam Cambridge, MA, USA,Cat. # ab32502), anti-BCL2 Ab (Abcam Cambridge, MA, USA, Cat. # ab117115), anti-Ki67 Ab (Abcam, Cambridge, MA, USA, Cat. # 16667), anti-Wee1 Ab (Cell Signaling, Beverly, MA, USA, Cat. # 4938 S), anti-AKT Ab and anti-p(Ser473)-AKT Ab (Cell Signaling, Beverly, MA, USA, Cat. # 2920 S and 4060 P), anti-cleaved Caspase 3 Ab (Cell Signaling, Beverly, MA, USA,Cat. # 9664), anti-CNTFRα Ab (Santa Cruz, USA. Cat. # sc-9993), goat anti-mouse IgG Fluor488 Ab, goat anti-rabbit IgG Fluor 546 Ab (Invitrogen, NY, USA, Cat. # A11017 and A11071), anti-GAPDH Ab and mouse and rabbit secondary Ab (Kangcheng, Shanghai, China. Cat. # KC-5G4, KC-MM-035, KC-RB-035).
Other reagents and kits used were: CCK8 (Dojindo, Japan, Cat. # CK04), Annexin V-FITC cell apoptosis assay kit, Cell cycle assay kit (Beyotime, Shanghai, China, Cat. #C1063and C1052), LY294002 (Cell Signaling, Beverly, MA, USA, Cat. #9901), 5-Aza-2-dC (Sigma Aldrich, Saint Louis, MO, USA, Cat. #A3656), CNTFRα siRNA (Santa Cruz, USA, Cat. #35076), SYBR real time kit (Takara, Dalian, China, Cat. #DDR041A), MSP-PCR kit (Epigentek, Farmingdale, NY, USA, Cat. # P-1016-80).
Other reagents and kits used were: CCK8 (Dojindo, Japan, Cat. # CK04), Annexin V-FITC cell apoptosis assay kit, Cell cycle assay kit (Beyotime, Shanghai, China, Cat. #C1063and C1052), LY294002 (Cell Signaling, Beverly, MA, USA, Cat. #9901), 5-Aza-2-dC (Sigma Aldrich, Saint Louis, MO, USA, Cat. #A3656), CNTFRα siRNA (Santa Cruz, USA, Cat. #35076), SYBR real time kit (Takara, Dalian, China, Cat. #DDR041A), MSP-PCR kit (Epigentek, Farmingdale, NY, USA, Cat. # P-1016-80).
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