Elastase congo red
Manufactured by Merck Group
Elastase Congo red is a laboratory equipment used for the detection and quantification of elastase activity. It is a colorimetric assay that utilizes the Congo red dye as a substrate for the enzymatic cleavage by elastase. The core function of this product is to provide a reliable and standardized method for the measurement of elastase levels in various biological samples.
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Lab products found in correlation
2 protocols using elastase congo red
1
Quantification of Virulence Factors in Pseudomonas aeruginosa
Check if the same lab product or an alternative is used in the 5 most similar protocols
(i) Elastase. P. aeruginosa supernatants (50 μL) were mixed with 20 mg (±0.2) of elastase Congo red (Sigma-Aldrich, Saint-Louis, MO) and 1 mL of Tris buffer (100 mM Tris, 1 mM CaCl2 [pH 7.2]), followed by incubation for 18 h at 37°C with shaking. The reaction was stopped with 100 μL of EDTA, followed by centrifugation, and the absorbance of the supernatants was measured at 490 nm and normalized to the A580.
(ii) Pyocyanin. Pyocyanin was extracted from 1 mL of cell-free culture supernatants with 1 mL of chloroform by vortexing. The chloroform phase was extracted with 500 μL of 0.2 N HCl. The absorbance of the aqueous phase was measured at 520 nm and normalized to the A580 (49 (link)).
(iii) Pyoverdine. Pyoverdine was quantified by spectrophotometry from cells grown in CAA, and the results are expressed as the A405/A580 ratio (124 (link)).
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2
Quantification of Virulence Factors in Pseudomonas aeruginosa
Check if the same lab product or an alternative is used in the 5 most similar protocols
(i) Elastase. P. aeruginosa supernatants (50 μL) were mixed with 20 mg (±0.2) of elastase Congo red (Sigma-Aldrich, Saint-Louis, MO) and 1 mL of Tris buffer (100 mM Tris, 1 mM CaCl2 [pH 7.2]), followed by incubation for 18 h at 37°C with shaking. The reaction was stopped with 100 μL of EDTA, followed by centrifugation, and the absorbance of the supernatants was measured at 490 nm and normalized to the A580.
(ii) Pyocyanin. Pyocyanin was extracted from 1 mL of cell-free culture supernatants with 1 mL of chloroform by vortexing. The chloroform phase was extracted with 500 μL of 0.2 N HCl. The absorbance of the aqueous phase was measured at 520 nm and normalized to the A580 (49 (link)).
(iii) Pyoverdine. Pyoverdine was quantified by spectrophotometry from cells grown in CAA, and the results are expressed as the A405/A580 ratio (124 (link)).
+ Expand
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