Anti 5his

SDS–PAGE was performed on Bio-Rad Mini-PROTEAN systems using standard protocols. For immunostaining, proteins were transferred onto 0.2-µm nitrocellulose membranes (Amersham Protran). Immunoblots were probed with primary antibodies and goat secondary antibodies coupled to alkaline phosphatase, and developed in alkaline buffer in the presence of 5-bromo-4-chloro-3-indolylphosphate and nitro-blue tetrazolium. The anti-HA (HA-7 clone, Sigma Aldrich), anti-FLAG (M2 clone, Sigma Aldrich), anti-StrepII (Sigma Aldrich), anti VSV-G (Sigma Aldrich) and anti-5His (Sigma Aldrich) monoclonal antibodies, and mouse secondary antibodies (Millipore) were purchased as indicated.

SDS-PAGE was performed on Bio-Rad Mini-Protean systems using standard protocols with homemade 12.5% polyacrylamide gels. For immunostaining, proteins were transferred onto 0.2-μm nitrocellulose membranes (Amersham Protran) with a Mini-Trans Blot cell (Bio-Rad). The membrane was then saturated in 5% milk and probed with primary antibodies. Mouse secondary antibody coupled to alkaline phosphatase was added and developed in alkaline buffer in the presence of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium. The antihemagglutinin (HA) (HA-7 clone; Sigma-Aldrich), anti-Flag (M2 clone; Sigma-Aldrich), anti-StrepII (Sigma-Aldrich), anti-5His (Sigma-Aldrich) monoclonal antibodies, and mouse secondary antibodies (Millipore) were purchased as indicated.