Bladder cancer cells, SCaBER (Squamous Cell Carcinoma (SCC); ATCC HTB-3) and UMUC3 (Transitional Cell Carcinoma (TCC); ATCC CRL 1749), were cultured in T-75 flasks (Cell Treat, Shirley, MA, USA) in EMEM (ATCC 30–2003) supplemented with 10% FBS (Peak Serum, PS FB-3) and 1% penicillin/streptomycin (Lonza). Cells were lifted using TrypLE Express (Gibco/Life Technologies, Grand Island, NY), centrifuged and plated into Costar strip well plates (Corning, Tewksbury, MA, USA) at 10,000 cells per well and cultured for 2 days prior to experimentation.
Costar strip well plates
Manufactured by Corning
Sourced in United States
Costar strip well plates are single-use polystyrene cell culture plates designed for a variety of laboratory applications. The plates feature individual wells that can be separated into strips, providing flexibility and efficiency in experimental setup and sample processing.
Automatically generated - may contain errors
3 protocols using costar strip well plates
1
Bladder Cancer Cell Culture Protocol
2
Culturing and Plating PC-3 Cells
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PC-3 (ATCC, CRL-1435) were cultured in T-75 flasks (Cell Treat, Shirley, Massachusetts) in Roswell Park Memorial Institute (RPMI) 1640 medium (Caisson, North Logan, Utah) supplemented with 10% fetal bovine serum (Peak Serum, Colorado) and 1% Penicillin/Streptomycin (Corning/Mediatech, Manassas, Virginia). Cells were lifted using TrypLE Express (Gibco/Life Technologies, Grand Island, New York), centrifuged, and plated into Costar stripwell plates (Corning, Tewksbury, Massachusetts) at 3.2 × 103 cells/cm2 for 24 hours prior to VD3 treatment or 48 hours prior to freezing.
3
Bladder Cancer Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bladder cancer cells, SCaBER (Squamous Cell Carcinoma (SCC); ATCC HTB-3) and UMUC3 (Transitional Cell Carcinoma (TCC); ATCC CRL 1749), were cultured in T-75 flasks (Cell Treat, Shirley, MA, USA) in EMEM (ATCC 30–2003) supplemented with 10% FBS (Peak Serum, PS FB-3) and 1% penicillin/streptomycin (Lonza). Cells were lifted using TrypLE Express (Gibco/Life Technologies, Grand Island, NY), centrifuged and plated into Costar strip well plates (Corning, Tewksbury, MA, USA) at 10,000 cells per well and cultured for 2 days prior to experimentation.
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