Purified Ly6G+ cells were resuspended in sterile PBS at a concentration of 1 × 105 cells/μl and loaded into a Hamilton syringe with a 30 G needle. In some cases, anti-NGF (Alomone) and/ or anti-IGF1 (Abcam) polyclonal antibodies, or polyclonal control antibodies (Sigma), were included in the cell suspension, at a final concentration of 1 μg/μl. 3 μl of cell suspension was injected into the posterior chamber of each eye of anesthesized mice on days 0 and 3 post ONC injury.
Polyclonal control antibodies
Manufactured by Merck Group
Polyclonal control antibodies are laboratory reagents used to validate the specificity and performance of immunoassays. They are a mixture of antibodies derived from different B cells, targeting multiple epitopes on an antigen. These antibodies serve as positive controls to ensure the reliability and consistency of experimental results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using polyclonal control antibodies
1
Purified Ly6G+ Cell Injection for ONC
2
Purified Ly6G+ Cell Injection for ONC
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