Anti tnf β

Anti-active caspase-3, -MMP-9, -MMP-13, -TNF-α and TNF-α were purchased from R&D Systems, Inc., (Heidelberg, Germany). Antibodies to phospho-specific p65 (NF-κB)/(Ser536) were purchased from Cell Technology (Beverly, MA, USA). Antibodies to collagen type II and alkaline phosphatase-linked sheep anti-mouse and sheep anti-rabbit secondary antibodies for immunoblotting were purchased from Millipore (Schwalbach, Germany). Anti-TNF-β was obtained from eBioscience (Frankfurt, Germany). Anti-Ki67 and secondary antibodies used for fluorescence labelling were purchased from Dianova (Hamburg, Germany). Anti-cyclooxygenase-2 was purchased from Cayman Chemical (Ann Arbor, MI, USA). Monoclonal anti-β-Actin was obtained from Sigma-Aldrich (Munich, Germany). Sox9 antibody was purchased from Acris Antibodies GmbH (Hiddenhausen, Germany). Polyclonal antibody against Sirt1 was purchased from Abcam PLC (Cambridge, UK).TNF-β and polyclonal rabbit Anti-TNF-β were obtained as described [40 (link)]. Bacteria-derived recombinant human TNF-α and LT-α (TNF-β), both purified to homogeneity with a specific activity of 50 million units/mg, were kindly gift from Genentech, Inc. (South San Francisco, CA).

Anti-CXCR4 (CXC-Motiv-Chemokinreceptor 4) was acquired from Abcam PLC (Cambridge, UK). Anti-CD133, -ALDH1, and -CD44 were purchased from Antibodies-online. Anti-phospho-specific p65 (NF-κB) and anti-MMP-9 were from R&D Systems (Heidelberg, Germany). Anti-E-cadherin, anti-vimentin, and anti-slug were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-FAK was purchased from Becton Dickinson (Heidelberg, Germany). Monoclonal antibodies to Ki-67 and secondary antibodies used for fluorescence labelling were purchased from Dianova (Hamburg, Germany). Anti-β-actin, alginate, resveratrol, and Staphylococcus aureus bacteria were from Sigma–Aldrich Chemie (Munich, Germany). TNF-β, TNF-α, anti-TNF-β-receptor, and anti-TNF-β were purchased from eBiosciences (Frankfurt, Germany). In addition, TNF-β and TNF-α, were kindly provided by Genetech, Inc. (South San Francisco, CA, USA) [34 (link)]. Secondary antibodies for Western blotting were from Millipore (Schwalbach, Germany) and gold particle-conjugated secondary antibodies were from Amersham (Braunschweig, Germany). resveratrol was dissolved in ethanol as stock and stored at –20 °C. Epon was from Plano (Marburg, Germany).