Horseradish peroxidase hrp conjugated anti rabbit and anti mouse secondary antibodies

SH-SY5Y neuroblastoma cells were purchased from American Type Culture Collection (ATCC, CRL-2266, Manassas, VA, USA). Manganese (II) chloride tetrahydrate (MnCl₂·4H₂O), DMEM and F-12, Fetal Bovine Serum (FBS), penicillin, streptomycin, and trypsin were supplied by Invitrogen (Carlsbad, CA, USA). Calpains, Calpeptin, SynaptoGreen^TMC4 (FM1-43), mouse α-Syn, synaptophysin, and β-actin primary antibodies were purchased from Sigma (Saint Louis, MO, USA). PrimeScript^®RT Enzyme Mix I and SYBR^®Premix Ex TaqTMII kits were provided by TaKaRa Biotech. Co., Ltd. (Dalian, China). Rabbit SNAP-25-C-terminal and mouse SNAP-25-N-terminal primary antibodies were purchased from Synaptic Systems (Goettingen, Germany). Rabbit Syntaxin 1 and VAMP-2 primary antibodies, and horseradish peroxidase (HRP) conjugated anti-rabbit and anti-mouse secondary antibodies were supplied by Abcam Ltd. (Hong Kong, China).

Monoclonal antibodies against phospho-AMPKα(thr172), phospho-STAT3(tyr705), phospho-ERK1/2(thr202/tyr204), phospho-p38(thr180/tyr182), p38, phospho-MEK1/2(ser221), MEK1/2, phospho-AKT(ser473), AKT, Bcl-2, Bax, p27, and polyclonal antibodies against phospho-mTOR(ser2448), mTOR, AMPKα, phospho-RSK(Thr359/Ser363), RSK, ERK1/2, LC3B, and Beclin 1 were purchased from Cell Signaling (Cell Signaling Technology, Inc., Beverly, MA, USA). Monoclonal antibodies specific to phospho-JNK(thr183/tyr185) and JNK, and polyclonal antibodies specific to STAT3 were purchased from Santa Cruz (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). Monoclonal antibody against Cyclin D1, polyclonal antibody against β-actin, and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse secondary antibodies were purchased from Abcam (Burlingame, CA, USA). Anti-phospho-p27(Thr198) polyclonal antibody was purchased from R&D (R&D Systems, Inc., Minneapolis, MN, USA).