Sybr gold for imaging

Manufactured by Thermo Fisher Scientific

SYBR Gold is a nucleic acid stain used for imaging and quantification of DNA and RNA in agarose gels and other applications. It is a sensitive fluorescent dye that binds to double-stranded DNA and single-stranded RNA, emitting green fluorescence upon excitation.

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Lab products found in correlation

Rnase a t1 mix, by Thermo Fisher Scientific (2 mentions) Gel doc imager, by Bio-Rad (2 mentions) Ssdna rna clean concentrate kit, by Zymo Research (2 mentions) Tp300024, by New England Biolabs (2 mentions) Ec6885box, by Thermo Fisher Scientific (2 mentions) Plasmid midiprep kit, by Qiagen (2 mentions)

Close spelling variants of this product

SYBR Gold (1016 citations)

2 protocols using sybr gold for imaging

Retron RT-DNA Purification and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retron RT-DNA was expressed in E. coli bMS.346 and purified in two steps. First, DNA was extracted from cells using a plasmid midiprep kit (Qiagen 12943). This purified DNA was then treated for 30 minutes at 37C with RNAse A/T1 mix (ThermoFisher EN0551) and, if required, DBR1 (OriGene TP300024) and/or RecJ_f (NEB M0264). This sample was then used as the input for the Zymo Research ssDNA/RNA Clean & Concentrate kit (Zymo D7011). Samples eluted from the ssDNA kit were resolved using TBE-urea PAGE (ThermoFisher EC6885BOX). Gels were stained with SYBR Gold for imaging (ThermoFisher S11494) and imaged on a Bio-Rad Gel Doc imager.

Bhattarai-Kline S., Lear S.K., Fishman C.B., Lopez S.C., Lockshin E.R., Schubert M.G., Nivala J., Church G, & Shipman S.L. (2022). Recording gene expression order in DNA by CRISPR addition of retron barcodes. Nature, 608(7921), 217-225.

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Retron RT-DNA Purification and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retron RT-DNA was expressed in E. coli bMS.346 and purified in two steps. First, DNA was extracted from cells using a plasmid midiprep kit (Qiagen 12943). This purified DNA was then treated for 30 minutes at 37C with RNAse A/T1 mix (ThermoFisher EN0551) and, if required, DBR1 (OriGene TP300024) and/or RecJf (NEB M0264). This sample was then used as the input for the Zymo Research ssDNA/RNA Clean & Concentrate kit (Zymo D7011). Samples eluted from the ssDNA kit were resolved using TBE-urea PAGE (ThermoFisher EC6885BOX). Gels were stained with SYBR Gold for imaging (ThermoFisher S11494) and imaged on a Bio-Rad Gel Doc imager.

Bhattarai-Kline S., Lear S.K., Fishman C.B., Lopez S.C., Lockshin E.R., Schubert M.G., Nivala J., Church G.M., & Shipman S.L. (2021). Recording gene expression order in DNA by CRISPR addition of retron barcodes.

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